TT2014 meeting report published in Transgenic Research

TT2014 meeting report published in Transgenic Research
TT2014 meeting report published in Transgenic Research

The meeting report of the 12th Transgenic Technology meeting (TT2014), held in Edinburgh on October 6-8, 2014, and organized by Douglas Strathdee, Peter Hohenstein and Bruce Whitelaw, has just been published in the scientific journal Transgenic Research.

TT2014 meeting report on the 12th Transgenic Technology meeting in Edinburgh: new era of transgenic technologies with programmable nucleases in the foreground.
Beck IM, Sedlacek R.
Transgenic Res. 2015 Feb;24(1):179-83.

The TT2014 meeting report has been written by Inken Beck and Radislav Sedlacek (Czech Centre for Phenogenomics, Institute of Molecular Genetics, Prague, Czech Republic), who will be responsible to organize the next (13th) Transgenic Technology meeting (TT2016) in Prague on March 20-23, 2016.

The TT2014 meeting Scottish style

The TT2014 meeting at the Assembly Rooms, Edinburgh, Scotland, UK, 6-8 October 2014
The TT2014 meeting at the Assembly Rooms, Edinburgh, Scotland, UK, 6-8 October 2014

The most popular ISTT meeting held to date took place last week in Edinburgh, UK, at the Assembly Rooms. More than 530 participants gathered in Scotland for the 12th Transgenic Technology (TT2014) meeting during three days, 6 to 8 October 2014, to discuss the latest advances in methods and applications for generating and analyzing genetically modified animals. The local Organizers, Douglas Strathdee, Peter Hohenstein and Bruce Whitelaw showed us how to run an splendid TT meeting Scottish style. The Organizing committee, helped by the In-Conference team and the ISTT council and Administration, should be praised for putting together an excellent collection of talks, covering the most up-to-date topics, where all participants could learn and share their experiences.

Janet Rossant awarded the 10th ISTT Prize at the TT2014 meeting in Edinburgh, for her outstanding contributions to the field of transgenic technologies. Janet Rossant appears in this picture with Alexandre Fraichard (left, genOway, CEO, sponsor of this award) and Lluis Montoliu (past-ISTT President, right)
Janet Rossant awarded the 10th ISTT Prize at the TT2014 meeting in Edinburgh, for her outstanding contributions to the field of transgenic technologies. Janet Rossant appears in this picture with Alexandre Fraichard (left, genOway, CEO, sponsor of this award) and Lluis Montoliu (past-ISTT President, right)

As many as 54 speakers were invited to present their research, including two keynote addresses (Rudolf Jaenisch, Alexandra Joyner), at the opening and clossing of the TT2014 meeting, two ISTT awarded scientists (Janet Rossant-10th ISTT Prize- and Feng Zhang-3rd ISTT Young Investigator Award), 26 invited speakers, 6 Showcase presentations from Scottish scientists, 5 short talks and 15 teasers selected from Poster abstracts. The impressive numbers of the TT2014 meeting continue with 176 Posters presented by participants attending the conference in Edinburgh and the many sponsors and exhibitors that generously contributed to the success of the 12th Transgenic Technology Meeting. The ISTT conference was followed by a hands-on workshop on basic zebrafish transgenic methods.

Lluis Montoliu, founder and past-ISTT President, awarded a sculpture representing a zygote, created by Bela Rozsnyay, by ISTT Board members for his dedicated work for the progress of the ISTT since inception of the Society and during the past 9 years (2006-2014). Lluis Montoliu appears next to Alison Cameron (ISTT Administrative Assistant) and Aimee Stablewski (ISTT Treasurer and Secretary)
Lluis Montoliu, founder and past-ISTT President, awarded a sculpture representing a zygote, created by Bela Rozsnyay, by ISTT Board members for his dedicated work for the progress of the ISTT since inception of the Society and during the past 9 years (2006-2014). Lluis Montoliu appears next to Alison Cameron (ISTT Administrative Assistant) and Aimee Stablewski (ISTT Treasurer and Secretary)

During the TT2014 meeting, at the corresponding ISTT General Assembly, the ISTT Members approved to dissolve the legal entity representing the Society in Spain, which has been substituted by the new legal entity, ISTT, Inc., a not-for-profit association registered in the New York State (USA) that from now onwards will be representing the interests of the Society. Also, during the course of the ISTT conference in Edinburgh, the ISTT Board members awarded Lluis Montoliu (Founder and Past-President of the ISTT, 2006-2014) and thanked his dedicated work for the ISTT, since its inception. Jan Parker-Thornburg is the new President of the ISTT.

The 13th Transgenic Technology Meeting, TT2016, will be held in Prague (Czech Republic) on 20-23 March 2016, organized by Radislav Sedlacek
The 13th Transgenic Technology Meeting, TT2016, will be held in Prague (Czech Republic) on 20-23 March 2016, organized by Radislav Sedlacek

The conference in Edinburgh concluded with the warm invitation by Radislav Sedlacek (IMG, Prague, Czech Republic), Organizer of the 13th Transgenic Technology Meeting, TT2016, to attend the next ISTT conference that will be held in Prague, Czech Republic, on 20-23 March 2016.

Good bye and thanks to Edinburgh! Hello and see you all again in Prague!

Welcome to Edinburgh, the city hosting the TT2014 meeting!

Welcome to Edinburgh, the city hosting the TT2014 meeting!
Welcome to Edinburgh, the city hosting the TT2014 meeting!

The 12th Transgenic Technology Meeting is about to begin this forthcoming weekend. On Sunday, 5 October, the Organizers have nicely prepared a welcome pre-meeting dinner to get together and meet all colleagues attending. The real TT2014 meeting‘s kick off will be on Monday morning, 6 October. During the following three days all delegates will have the pleasure (and the privilege, owing the great interest triggered by this conference, absolutely sold out) to listen and discuss the latest advances in transgenic technologies, through lectures delivered by the key players in our field, the ones that have developed or contributed to disseminate the use of the newest tools, the most updated experimental strategies applied to generate and analyze genetically modified animals. More than 530 participants will fill the Assembly Rooms in the historic city of Edinburgh. This TT meeting has become not only the most popular ISTT conference organized to date but also the TT meeting where the highest number of posters (176) will be shown. Therefore, besides enjoying the talks, all TT2014 delegates will have a unique opportunity to browse through the work of dozens of many other laboratories and facilities, willing to share their advances, problems encountered, solutions found and innovative proposals.

The International Society for Transgenic Technologies (ISTT) is proud to have chosen Edinburgh as the venue for TT2014 and, hence, wants to congratulate the Organizing Team, led by Douglas Strathdee, Chair of the conference, along with Peter Hohenstein and Bruce Whitelaw, co-chairs, the technical secretariat provided by In-Conference and the rest of members of the Organizing committees, for having prepared what surely will be another great TT meeting, as all precedent meetings were. The TT2014 meeting will be followed by a practical workshop on Zebrafish Transgenic Techniques, where a limited number of participants will have the opportunity to meet experts in the field and learn the basic techniques applied nowadays to use this vertebrate as an alternative animal model to study gene function or disease.

If you are already in Edinburgh, enjoy the city! If you are travelling to Edinburgh: have a safe trip! If, however, and unfortunately, you have not managed to attend this time, but you are a member of the ISTT, please wait to grasp some of the excellence and talent that will be presented during next week through the talks that we will be at the members-only area, after obtaining the consent from the corresponding speakers. Also, life goes on, and ISTT is already planning for the next TT meeting, already launched: the 13th Transgenic Technology Meeting (TT2016) that will be held in Prague (Czech Republic) in March 2016, organized by Radislav Sedlacek.

See you all soon in Edinburgh!

 

CRISPR-Cas resources at the ISTT web site

CRISPR-Cas resources at the ISTT web site. Illustration from: CRISPR-Cas technology and applications (video by Le Cong from Feng Zhang's lab at MIT/BROAD institute. This video has been made possible thanks to Addgene)
CRISPR-Cas resources at the ISTT web site. Illustration from: CRISPR-Cas technology and applications (video by Le Cong from Feng Zhang’s lab at MIT/BROAD institute. This video has been made possible thanks to Addgene)

CRISPR-Cas strategies are discussed and applied everywhere for the targeted modification of genomes. Every day a new lab is considering using CRISPR-Cas for the generation of their subsequent animal mutants. There are many papers regularly being published on this subject and a number of excellent online resources have been prepared by several labs actively working in this field. For newcomers/beginners this might seem (and it is) a lot of information, potentially difficult to digest.

In this regard, from the International Society for Transgenic Technologies (ISTT), we have prepared a simple web page containing a selection of CRISPR-Cas resources and related information, for anyone willing to start using these technologies. Please, feel free to suggest any additional CRISPR-Cas resources you might be aware/using/have developed, which might be worth including in these web pages. Thanks.

In particular, we wish to thank Feng Zhang (MIT/BROAD) and his lab for their efforts, videos (such as this video from Le Cong, PhD, illustrating this post, this video has been made possible thanks to Addgene, and is part of this series), online desing tools, protocols, etc… disseminating these technologies. Feng Zhang will receive the third ISTT Young Investigator Award and will attend the TT2014 meeting in Edinburgh.

TT2014 abstracts: submission deadline is approaching (30 June)

TT2014 abstracts: submission deadline is approaching (30 June)
TT2014 abstracts: submission deadline is approaching (30 June)

From the International Society for Transgenic Technologies (ISTT) we warmly invite and encourage you all to submit your most recent and exciting results and developments in animal transgenesis to be presented at the forthcoming 12th Transgenic Technology (TT2014) meeting, which will be held in Edinburgh, Scotland, UK, on 6-8 October 2014. Deadline for submitting abstracts for the TT2014 meeting is June 30.
To submit an abstract please visit this TT2014 meeting web page.

All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Authors are requested to submit an abstract with the following requirements:

  • Title (max. 25 words)
  • Name authors and affiliations (first author is the presenting author).
  • Text of the communication (max. 400 words).
  • Abstracts should be submitted no later than June 30, 2014.

Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

Posters
Posters will be on display in the exhibition area throughout the duration of the meeting. Poster boards are 1.00m wide x 2.00m high and we recommend posters do not exceed 1.50m in length. A supply of Velcro tabs will be available at the venue. No screws or double-sided adhesive tape will be allowed due to the damage they can cause to the boards.

Best Poster Awards
All posters presented at the TT2014 meeting will be eligible for one of the ISTT Best Poster Awards, generously sponsored by Charles River, Inc.

Oral Presentations
A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Should you be interested in being considered to speak at the meeting please select the appropriate option when submitting your abstract.

Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare

We are looking forward to receiving your exciting works to discuss the latest development on animal transgenesis!. See you all in Edinburgh!

The TT2014 Registration Awards for ISTT members: deadline for submitting applications is 30th June

The TT2014 Registration Awards for ISTT members: deadline for submitting applications is 30th June
The TT2014 Registration Awards for ISTT members: deadline for submitting applications is 30th June

A minimum of six registration awards for ISTT members willing to attend the 12th Transgenic Technology (TT2014) meeting, to be held in Edinburgh, Scotland, UK, on 6-8 October 2014, will be sponsored by the International Society for Transgenic Technologies (ISTT). Applications should be sent, along with the registration confirmation and the requested additional documents to istt@transtechsociety.org by June 30, 2014. The ISTT will pay the Registration Fee of all applicants selected for one of these TT2014 registration awards. Please note that applicants not selected for an award are required to pay the corresponding registration fee. Please note the Award covers registration fees and attendance to all social events, however, does not cover travel, accommodation expenses or attendance at pre meeting events. Award decisions will be communicated by July 15, 2014 and awardees will receive a diploma at the TT2014 Meeting.

Applications for registration awards by ISTT members should be sent to the official ISTT email address (istt@transtechsociety.org), by June 30, 2014, and should include the following documentation:

  • Applicant’s CV
  • Letter describing how the applicant will benefit from attendance at the TT meeting
  • Only for technician/students: Letter of support from the applicant’s head/supervisor stating the support to the applicant’s participation in the TT meeting and highlighting why attendance will benefit the applicant’s career

Procedure
Applicants must register first at the TT2014 website and select, as a payment method, “application for registration awards”. The ISTT will pay the Registration Fee of all applicants selected for an award. However, if they are not selected they would be kindly requested to pay the corresponding registration fee

Selection Process
The ISTT Council will examine all applications received. Registration Awards will be selected according to the documents submitted and with preference given to:

  1. Student/Technician ISTT Members
  2. ISTT Members submitting an abstract for presentation as a poster/short-oral communication at the TT meeting
  3. Any other ISTT Member

Decision
Award decisions will be communicated by July 15, 2014. Awardees will have their TT registration fees entirely waived. Awardees will receive a diploma at the Awards ceremony at the end of the TT2014 meeting. Applicants that are not yet members of the ISTT may choose to register/join first the ISTT and simultaneously submit their travel/registration award application. ISTT Registration/Travel awards for each TT meeting will only be provided to ISTT members who have paid their current annual fees.

The TT2014-associated Zebrafish Transgenesis Workshop: 8-10 October 2014 in Edinburgh

The TT2014-associated Zebrafish Transgenesis Workshop: 8-10 October 2014 in Edinburgh
The TT2014-associated Zebrafish Transgenesis Workshop: 8-10 October 2014 in Edinburgh

Immediately after the 12th Transgenic Technology (TT2014) meeting, a unique hands-on zebrafish transgenesis workshop will be taking place in Edinburgh, on October 8-10, 2014. This will be the first non-rodent hands-on transgenic workshop ever organized after a TT meeting. This workshop is offered to willing participants registered to attend the TT2014 meeting (October 6-8, 2014). Registration is handled separately and, at first, intended participants should submit their expression of interest to Organizers. Please note that attendance at this meeting is limited to 20 participants only. Due also to high demand for places, there is a selection process in place for all applicants wishing to attend the workshop. Applications should be sent, along with proof of registration to the following email address: zebrafish_tt2014@transtechsociety.org by June 30, 2014. Decisions on the successful applicants will be communicated by July 15, 2014.

The zebrafish is fast becoming a very significant vertebrate animal model system in scientific research; highlighted by the exponential increase in zebrafish-related publications. The role of zebrafish in science is only going to increase in the future; techniques are being constantly developed and more and more people recognise its advantages and benefits to research.

This small freshwater, tropical fish has some clear and unique advantages as a model system; these include its optical transparency, with ex-utero synchronous and rapid development, which allows direct, and continuous in-vivo observation. Genetic and pharmacological manipulation is relatively straight forward and the zebrafish is able to regenerate all organs and tissues. The fish can be maintained under very economic conditions and can be obtained in significant numbers, allowing statistically high N values. Another major advantage is that the developing zebrafish can survive for several days without a cardiovascular system, allowing examination of embryonic-lethal genotypes and phenotypes, from the single-cell stage. Whilst the zebrafish may lack certain (and some obvious) mammalian organs, and despite being physically very different, there are very important genetic and cellular similarities, with development being directly comparable to humans.

The workshop ‘An Introduction to Zebrafish Transgenesis’ follows TT2014 and is the first practical workshop in a non-rodent model species. The practical sessions will start with gene expression interference using classical morpholino microinjection on Day 1, which is complemented on Day 2 with microinjection of a simple fluorescent transgene construct. The scheduled embryology sessions are separated by a wide-ranging series of talks from leading experts in aquatic transgenics. Both the tutorials and practical instruction will provide all the information needed to perform husbandry and gene manipulation in the early zebrafish embryo. Topics include: husbandry best practise, microinjection principles, transgenesis and mutagenesis using recombinases, imaging and cryopreservation.

Appointed teachers and instructors for this zebrafish workshop include: Liz Patton, Carl Tucker, Koichi Kawakami, Stephen Ekker, Keith Joung, Henry Roehl, Robert Kelsh, Martin Denvir, David Lyons, Dirk Seiger, Yi Feng and Karthikeyani Paranthaman.

If you are interested to learn how to work with this alternative vertebrate animal model do not miss this extraordinary opportunity to become familar with basic zebrafish transgenesis techniques and register your interest to attend this hands-on workshop, associated with the TT2014 meeting.

 

Highlights of the TT2014 meeting in Edinburgh: a conference you can’t miss!

The TT2014 meeting in Edinburgh (6-8 October 2014)
The TT2014 meeting in Edinburgh (6-8 October 2014)

This year’s ISTT main activity is the 12th Transgenic Technology conference, the TT2014 meeting, which will be held in Edinburgh, Scotland, UK, on 6-8 October 2014, followed by a 2-day hands-on workshop on basic zebrafish transgenesis techniques. The ISTT promotes the TT meetings every 18 months, these being the most important activity of our Society. This year, the local Organizers and advisory committees, commanded by Douglas Strathdee, need to be praised for preparing a most appealing and interesting program, addressing hot topics, current and most up-to-date issues actively discussed nowadays by the transgenic animal community. Talks that will be presented by the most active and prestigious scientists in our field.

Why you shouldn’t miss the TT2014 meeting?

  • If you are interested in the new transgenic methods associated to nucleases (ZFNs, TALENs and CRISPRs-Cas9) there will be plenty of interesting talks where these new fantastic tools will be presented and discussed, directly by the key players in this rapidly-evolving field, including: Rudolf Jaenisch, William Skarnes, Angelika Schnieke, Kai Schönig, Ignacion Anegon, Pawel Pelczar, Francis Stewart, Keith Joung and Feng Zhang. And, most likely, these techniques will be referred and cited in many additional talks too, including the round table discussion about the future of transgenic core facilities, chaired by James Bussell.
  • If you are interested in ES cell biology and in innovative uses of ES cells and associated technologies there will be unique talks delivered by Jos Jonkers, Austin Smith, Ian Chambers, Janet Rossant and Alex Joyner
  • If you are interested in phenotyping your mouse animal models there will be fantastic talks delivered by Jacqueline White, Stephen Murray, David Adams, Daniel Murphy, Anna-Katerina Hadjatonakis and Vasilis Ntziachristos
  • If you are interested in non-rodent, large mammals and birds, animal models there will be great talks by James Murray, Angelika Schnieke, Mike McGrew and Adrian Shermann
  • If you are interested in rats there will be compelling talks by Kai Schönig and Ignacio Anegon
  • If you are interested in zebrafish animal models there will be fascinating talks by Stephen Ekker, Koichi Kawakami, Keith Joung and Elizabeth Patton
  • If you are interested in animal welfare and 3Rs, in the best use of our laboratory animals, there will be captivating talks by Peter Hohenstein, Sara Wells and Jan-Bas Prins.

Therefore, there will be really engaging talks interesting to everyone in our field. This is why you shouldn’t miss this great and unique opportunity!.

Register now for the TT2014 meeting. Submission of abstracts will be accepted up to June 30. Early-Bird registration at reduced fees will be promoted up to July 31. ISTT members are entitled to reduced fee registration.

See you all in Edinburgh in October!

 

SALAAM: Sharing Advances on Large Animal Models

SALAAM: Sharing Advances on Large Animal Models
SALAAM: Sharing Advances on Large Animal Models

The EU-COST action SALAAM (Sharing Advances on Large Animal Models) was launched yesterday in Brussels, at a kick-off meeting attended by most of its members. This 4-year EU-COST action is currently formed by 17 countries and more than 44 participants, including many experts in the fields of animal genetics, physiology, transgenesis, bioethics, welfare and animal science, with a focus on large (i.e. non-rodent) animal models. This EU-COST action is chaired by Prof Eckhard Wolf (Germany) and vice-chaired by Dr. Pascale Chavatte-Palmer (France) and it includes various ISTT members such as Bruce Whitelaw (UK), Zsuzsanna Bosze (Hungary), András Dinnyes (Hungary), Cesare Galli (Italy) and Lluis Montoliu (Spain). In addition, another participant in this EU-COST action, Angelika Schnieke (Germany) is one of the invited speakers at the forthcoming 12th Transgenic Technology (TT2014) meeting to be held in Edinburgh (Scotland, UK).

EU-COST (European Cooperation in Science and Technology) is one of the oldest European initiatives in Science, an intergovernmental framework for European Cooperation in Science and Technology, allowing the coordination of nationally-funded research on a European level. SALAAM EU-COST action, as its acronym indicates, aims to sharing advances in genetic engineering and phenotyping of non-rodent mammals to develop predictive animal models for translational medicine. While recognizing the value of small and most popular animal models (mouse, rat, zebrafish, Drosophila, C. elegans, …) and its powerful genetics for increasing our knowledge on complex biological systems and for proof-of-concept-type experiments, this EU-COST action SALAAM focuses on large (i.e. non-rodent) mammalian models, since these may bridge the gap between proof-of-concept studies and more effective clinical trials, leading to better translational animal models for the study of human diseases. The research projects undertaken using rodent and non-rodent animal models should not be perceived as competition or opposed initiatives, rather as complementary studies, where each animal species is selected according to its particular value and expected benefits for the ultimate goal, that is, our understanding on the function of the mammalian (i.e. human) genome and the eventual development of effective treatments for many human diseases. During the course of this EU-COST action several conferences and training workshops will be organized, open to anyone interested in the field, to discuss about (1) new technologies (including the application of genome editing nucleases, i.e. CRISPR-Cas, for the generation of improved genetically altered animal models); (2) defining best animal models for specific phenotyping studies; (3) creation of databases for sharing information on animal models creates, tissues available and protocols; and (4) animal welfare, bioethics and communication to the public. All these conferences and training courses will be adequately advertised through the ISTT web site.

At the International Society for Transgenic Technologies (ISTT) we care about the generation and the analysis of “all” genetically altered animals, not only focused in the classical rodent models, but also including the work done with other species, with large animal models, in livestock. In this regard, the ISTT has been traditionally supporting conferences on non-rodent transgenic animals, organized in Tahoe by ISTT Member Jim Murray (UC Davis, USA) and has promoted a web page within the ISTT web site where most of the advances on livestock and other non-rodent genetically modified animal resources are shared. At the next 12th Transgenic Technology (TT2014) meeting, which will be held in Edinburgh on 6-8 October 2014, the Conference Organizers (Douglas Strathdee-Chair, Peter Hohenstein and Bruce Whitelaw) have scheduled a session on animal biotechnology, where the recent work accomplished using large animal models will be discussed. In addition, immediately following the TT2014 meeting, a hands-on workshop on zebrafish transgenesis methods will be offered to interested participants.

Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June
Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

The submission of abstracts for the 12th Transgenic Technology (TT2014) meeting, to be held in Edinburgh (Scotland, UK), on 6-8 October, is OPEN. All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Authors are requested to submit an abstract with the following requirements: Title (max. 25 words), Name authors and affiliations (first author is the presenting author), and, Text of the communication (max. 400 words). Abstracts should be submitted no later than June 30, 2014. Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

Posters
Posters will be on display in the exhibition area throughout the duration of the meeting. Poster boards are 1.00m wide x 2.00m high and we recommend posters do not exceed 1.50m in length. A supply of Velcro tabs will be available at the venue. No screws or double-sided adhesive tape will be allowed due to the damage they can cause to the boards. All presented Posters at the TT2014 meeting will be entitled to the Best Poster Awards, generously sponsored by Charles River.

Oral Presentations
A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Should you be interested in being considered to speak at the meeting please select the appropriate option when submitting your abstract.

Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare