Ultra-superovulation in C57BL/6 mice: 100 oocytes/female

New achievement in Reproductive Biology by Toru Takeo & Naomi Nakagata (CARD, University of Kumamoto, Japan). Ultra-superovulation of C57BL/6 mice: 100 oocytes/female obtained priming the animals with equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS)
New achievement in Reproductive Biology by Toru Takeo & Naomi Nakagata (CARD, University of Kumamoto, Japan). Ultra-superovulation of C57BL/6 mice: 100 oocytes/female obtained priming the animals with equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS)

Last September, in Buffalo (USA), at the CARD-RPCI Mouse Sperm and Embryo Cryopreservation course organized by Naomi Nakagata, Aimee Stablewski and Jan Parker-Thornburg, Naomi Nakagata himself presented the preliminary results of an amazing achievement in Reproductive Biology they had accomplished at the University of Kumamoto (Japan), namely: the obtention of more than 100 oocytes per C57BL/6 female after devising a new protocol for superovulation, a method they introduced as ULTRA-superovulation. Now, these totally unexpected results see the light in the form of a scientific manuscript, published yesterday in the PLOS ONE journal:

Toru Takeo & Naomi Nakagata (2015) Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice. PLOS ONE, Published: May 29, 2015DOI: 10.1371/journal.pone.0128330

In brief, in this publication, Toru Takeo and Naomi Nakagata describe their superovulation results using young (4-weeks old) C57BL/6 female after envisaging a new priming protocol. The combined used of equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS), in a protocol they call IASe treatment, significantly increased the number of oocytes obtained per C57BL/6 females. On average, more than 100 oocytes/female were obtained, about 3-4 times the number of oocytes regularly obtained by classical superovulation protocols. Thereafter, the authors tested the quality of these oocytes and used them for IVF, obtaining high fertilization rates (~90%), comparable to the high values regularly obtained with the new CARD methods these authors also devised recently, which have boosted the field of cryopreservation of mutant mice. Furthermore, the authors verified that the number of pups obtained after transferring all these embryos, obtained from IASe-derived oocytes and IVF into recipients, was also 2-3 times higher.

The CARD YouTube channel has also released a movie showing ampullas of oviducts from IASe-treated C57BL/6 females literally full of oocytes.

The IAS reagent used by Takeo & Nakagata is not yet commercially available. In the paper, the authors produced the IAS by themselves and titrated the product until finding the optimal dose required for maximum output. On the contrary, eCG is commercially available and is commonly used in all mouse reproductive biology and transgenic labs to promote follicle growth. Subsequent experiments will be needed to explore the validity of these results in other mouse strains and species. In addition, a commercial reliable and validated source of IAS will greatly facilitate the dissemination of this new ultra-superovulation method among the scientific community. It is also remarkable to note that the application of the IASe treatment will logically reduce the number of superovulated donor females required to obtain oocytes for cryopreservation/IVF purposes, as nicely demonstrated in this first publication, and, likely, for other aims (i.e. microinjection of DNA or RNA/genome editors to produce genetically altered mice).

Congratulations once again to Toru Takeo and Naomi Nakagata for these impressing results and for their new spectacular achievement in mouse reproductive biology!.

CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course, Roswell Park Cancer Institute, Buffalo, NY, USA, September 15th-19th, 2014

CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course, Buffalo, NY, USA, September 15-19, 2014
CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course, Buffalo, NY, USA, September 15-19, 2014

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor another CARD Cryopreservation Course. This time, for the first time, the CARD methods will be taught in North America: the CARD-RPCI Mouse Sperm and Embryo Cryopreservation Practical Course will be held at Roswell Park Cancer Institute (RPCI) in Buffalo, NY USA. The course will be held September 15th-19th, 2014, and will be a hands-on intensive workshop.

The course is organized by Naomi Nakagata (CARD-Kumamoto University, Japan, Coordinator of CARD), Aimee Stablewski (Roswell Park Cancer Institute, Buffalo, NY, USA, Co-Director of the Gene Targeting and Transgenic Resource) and Jan Parker-Thornburg (MD Anderson, Houston, TX, USA, Director of the Genetically Engineered Mouse Facility).

This course is open to anyone interested. Pre-application will be required, including, at least, a recent CV, completion of this application questionnaire, and a letter prepared by the intended participant describing how the applicant will benefit by attending this course and how relevant is the course material to his/her work. Additional documents are welcome, at the discretion of participants, including supporting letters by supervisors (where appropriate), reference letters, etc… Pre-applications should be submitted by email to: buffalo_card_cryocourse@transtechsociety.org

The maximum number of participants attending this course will be 18, distributed among countries and institutions, and according the documentation provided and the interests expressed. Pre-applications will be accepted starting April 1st, 2014 and will close on May 31st, 2014. The review and selection of participants will be done by the Organizers from June 1st-15th, 2014. Registrations and payments for selected participants will be accepted from June 16th, 2014 to August 31st, 2014. If required, an ordered waiting list will be prepared and any cancellation or unpaid registration by August 31st, 2014 will be readily substituted by the first available person from this waiting list, starting on September 1st, 2014.

The course registration fee is $1300 USD (with a reduced fee of $1100 USD for ISTT members). This fee includes participation in the entire course, all materials and reagents, lunches over the 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, if required, at a hotel on Roswell Park Cancer Institute’s campus, where all instructors and lecturers will be also lodged, hence further promoting interaction from breakfast to dinner. The official language of the course will be English.

COURSE INFORMATION: Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have boosted the mouse cryopreservation field with improved methods for fresh and frozen sperm techniques and associated optimized IVF methods that have resulted in unparalleled increased efficiencies for the cryopreservation and rescue of relevant mouse lines.

The aim of this course is to introduce the new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities and willing to implement these new methods, directly taught by the team which devised them. Each participant will have one stereomicroscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field. The number of instructors and lecturers appointed is 17.

Hands-on topics that will be covered during this cryopreservation course:

  • Isolating unfertilized mouse oocytes
  • Isolating and cold storage/shipping of mouse cauda epididymis
  • Isolating and cold storage of embryos
  • Freezing/thawing mouse sperm and IVF using CARD frozen sperm and legacy sperm
  • Fresh mouse sperm and IVF
  • Cold Storage sperm and IVF
  • Freezing/thawing 2-cell IVF-derived mouse embryos
  • Vitrification of mouse oocytes and embryos
  • IVF of vitrified mouse oocytes
  • Ovary transplantation/ovary freezing
  • Embryo transfer techniques in mice (oviduct, uterus via NSET)

Additional lectures on the following topics:

  • Historic and Scientific perspectives of embryo and sperm cryopreservation
  • Comparing current embryo and sperm cryopreservation methods
  • Vitrification of oocytes and their use for IVF
  • New US Guidelines for the use of animals in research/IACUC
  • Cold storage and transport of germplasm
  • Large archiving and distribution centers- challenges and solutions
  • Shipping mice, refrigerated and frozen material
  • CRISPR/Cas9
  • Freezing and transplantation of ovaries
  • NSET: non-surgical embryo transfer
  • Breeding strategies for cohort generation of GEM’s
  • CARD

Instructors:

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Shuuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Kiyoko Fukumoto (CARD-Kumamoto University, Japan)
  • Yukie Haruguchi (CARD-Kumamoto University, Japan)
  • Tomoko Kondo (CARD-Kumamoto University, Japan)
  • Yumi Takeshita (CARD-Kumamoto University, Japan)
  • Yuko Nakamuta (CARD-Kumamoto University, Japan)
  • Tomoko Umeno (CARD-Kumamoto University, Japan)
  • Jan Parker-Thornburg (MD Anderson, Houston, TX, USA)
  • Aimee Stablewski (Roswell Park Cancer Institute, Buffalo, NY, USA)
  • Dawn Barnas (Roswell Park Cancer Institute, Buffalo, NY, USA)
  • Amar Dasani (Taconic, Germantown, NY, USA)
  • Andrea Dunn (Taconic, Germantown, NY, USA)
  • Kristy Williams (USA)
  • Barbara Stone (ParaTechs, Lexington, KY, USA)
  • Jorge Sztein (Barcelona, Spain)

Additional lectures by:

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Sandra Sexton (Roswell Park Cancer Institute, Buffalo, NY, USA)
  • Jorge Sztein (Barcelona, Spain)
  • Barbara Stone (ParaTechs, Lexington, KY, USA)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)
  • Carlisle Landel (Transposagen Inc., Lexington, KY, USA)
  • Amar Dasani (Taconic, Germantown, NY, USA)

CARD-CNB Cryopreservation Course Report

Organizers, instructors, lecturers and participants at the CARD-CNB Cryopreservation Course, held at CNB-CSIC in Madrid, Spain, on 7-11 October 2013 and organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain)
Organizers, instructors, lecturers and participants at the CARD-CNB Cryopreservation Course, held at CNB-CSIC in Madrid, Spain, on 7-11 October 2013 and organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain)

This past week, 7-11 October 2013, the CARD-CNB Mouse Sperm and Embryo Cryopreservation Course was held at CNB-CSIC, in Madrid, Spain, with great success and accompanied with also great sunny weather. This was the first cryopreservation course of this kind, co-organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain), were the newest methods developed by CARD, at the Nakagata lab, were demonstrated in Europe, directly by the CARD team. The instructors at this CARD-CNB course were commanded by the CARD-University of Kumamoto team, from Japan (Toru Takeo, Kiyoko Fukumoto, Tomoko Kondo, Yukie Haruguchi, Yumi Takeshita, Yuko Nakamuta and Shuuji Tsuchiyama), and additional help and collaboration was provided from the Mouse Biology Program at UC-Davis, CA, USA (Kristy Kinchen), from INIA, Madrid, Spain (Raúl Fernández), from CIEMAT, Madrid, Spain (Jesús Martínez), from USA (Jorge Sztein), from Paratechs, Lexington, KY, USA (Barbara Stone) and from CNB-CSIC (Julia Fernández, María Jesús del Hierro, Marta Castrillo and Isabel Martín-Dorado), for several of the methods demonstrated. All instructors must to be praised for their deep knowledge, patience and extraordinary dedication and commitment towards the success of this course. Complementary and most interesting lectures were provided on a wide variety of topics related to the course main focus, including: animal welfare and regulations by Belén Pintado and Jorge Guillén; the history, fundaments and comparison of methods by Jorge Sztein; the effects of the in vitro culture of mouse embryos by Alfonso Gutiérrez-Adán; safety and handling issues of liquid nitrogen by Jesús Martínez; shipping frozen and refrigerated materials by Toru Takeo, databases in a cryopreservation lab, by Shuuji Tsuchiyama, about EMMA by Lluis Montoliu and CARD by Naomi Nakagata, as examples of mouse embryo and sperm archives, and, also, on the new editing nucleases for genome modification, by Kai Schönig (Mannheim, Germany), a talk sponsored by Sigma.

As many as 24 participants, coming from research institutions or companies located in 16 countries around the world (UK, Spain, Australia, USA, Canada, Czech Republic, Brazil, Finland, France, Denmark, Israel, Netherlands, Portugal, Sweden, Italy and Taiwan) were presented with the latest advances in mouse sperm and embryo cryopreservation and all associated mouse reproductive biology ancillary techniques.

The topics covered by the course included the following major areas: obtaining sperm from mouse cauda epididymis, obtaining unfertilized mouse oocytes, three different types of in vitro fertilization techniques (using fresh, frozen or refrigerated sperm), vitrification of unfertilized oocytes and 2-cell embryos (freezing and thawing), slow-method for freezing and thawing 2-cell embryos, refrigerated sperm and 2-cell embryos, abdominal and scrotal vasectomies, three types of embryo transfer (oviduct, uterus and non-surgical, with NSET tools), freezing and thawing mouse sperm and ICSI, among many additional common methodologies used to handle mouse embryos and gametes adequately.

The course was very intensive, but the kind atmosphere created by participants and instructors was excellent and, hence, all the tight and carefully devised demonstrations and practices, packed within a very busy schedule, could be run smoothly and successfully without problems. The vast experience in running this type of cryopreservation courses and the remarkable professionality of our colleagues from CARD-University of Kumamoto were key for the accomplished success. All methods followed their three-step learning process. At first, the theory and fundaments were briefly provided and summarized. Then, the method was demonstrated by instructors and, finally, the participants executed the procedures by themselves, with the help of instructors.

The participants left this cryopreservation course to return to their countries and institutions with the most satisfactory results obtained and with plenty of new information to digest, process and reproduce. All participants were given the task to spread the word and disseminate the use of these highly efficient and robust cryopreservation techniques that have boosted the field.

This CARDCNB cryopreservation course was sponsored by the International Society for Transgenic Technologies (ISTT) and received the co-sponsorship and support from a number of companies whose contributions need to be greatly acknowledged as well: Leica, Charles River, Sigma, Labotect, Cosmo-Bio, Kyudo, Harlan and Paratechs.

CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013
CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course that will be held at the National Centre for Biotechnology (CNB-CSIC), in Madrid, CSIC, during one week, on 7-11 October 2013, organized by Naomi Nakagata (CARD-Kumamoto University, Japan, Coordinator of CARD) and Lluis Montoliu (CNB-CSIC, Madrid, Spain, Coordinator of the Spanish EMMA node).

This course is open to anyone interested. Pre-application will be required, including, at least, a recent CV and a letter prepared by the intended participant describing how the applicant will benefit by attending this course and how relevant is the course material to his/her work. Additional documents are welcome, at the discretion of participants, including supporting letters by supervisors (where appropriate), reference letters, etc… Pre-applications should be submitted by email to cryocourse@cnb.csic.es by 31 May 2013.

The maximum number of participants attending this course will be 20, distributed among countries and institutions, and according the documentation provided and the interests expressed. Pre-applications will be accepted until 31 May 2013. The review and selection of participants will be done by the Organizers from 1 to 15 June 2013. Registrations and payments for selected participants will be accepted from June 16, 2013 to August 31, 2013. If required, an ordered waiting list will be prepared and any cancellation or unpaid registration by 31 August 2013 will be readily substituted by the first available person from this waiting list, starting on 1 September 2013.

The course registration fee is 800 Euros (with a reduced fee of 750 Euros for ISTT Members). This fee includes participation in the entire course, all materials and reagents, lunches over the 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, if required, at a convenient nearby hotel, close to CNB Campus, where all instructors and lecturers will be also lodged, hence further promoting interaction from breakfast to dinner. The official language of the course will be English.

COURSE INFORMATION: Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have boosted the mouse cryopreservation field with improved methods for fresh and frozen sperm techniques and associated optimized IVF methods that have resulted in unparalleled increased efficiencies for the cryopreservation and rescue of relevant mouse lines. At the CNB-CSIC in Madrid, hosting the Spanish EMMA node, these new CARD cryopreservation methods have been successfully implemented and, moreover, a fruitful collaboration has been established with the laboratory of Prof. Naomi Nakagata, eventually resulting in the signature of an institutional cooperation agreement between the CSIC and the University of Kumamoto, under the framework of which this cryopreservation course is organized. The aim of this course is to introduce the new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities and willing to implement these new methods, directly taught by the team which devised them. Each participant will have one stereomicroscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field. The number of instructors and lecturers appointed is 20.

Hands-on topics that will be covered during this cryopreservation course

  • making pipettes and practising embryo handling
  • isolating unfertilized mouse oocytes
  • isolating and cold storage/shipping of mouse cauda epididymis
  • freezing/thawing mouse sperm and IVF
  • fresh mouse sperm and IVF
  • freezing/thawing 2-cell IVF-derived mouse embryos
  • vitrification of mouse oocytes and embryos
  • embryo transfer techniques in mice
  • vasectomy of male mice
  • demonstration of ICSI

Additional lectures on the following topics

  • new EU Directive on the protection of animals for experimentation
  • new US Guidelines for the use of animals in research
  • epigenetic effects of in vitro culture on mouse embryos
  • databases for handling information in cryopreservation facilities
  • shipping mice, refrigerated and frozen material
  • safety issues handling liquid nitrogen in a cryopreservation facility
  • EMMA and Infrafrontier-I3
  • MMRRC

Instructors

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Kiyoko Fukumoto (CARD-Kumamoto University, Japan)
  • Tomoko Kondo (CARD-Kumamoto University, Japan)
  • Yukie Haruguchi (CARD-Kumamoto University, Japan)
  • Yumi Takeshita (CARD-Kumamoto University, Japan)
  • Yuko Nakamuta (CARD-Kumamoto University, Japan)
  • Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Raul Fernández (INIA, Madrid, Spain)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)
  • Julia Fernández (CNB-CSIC, Madrid, Spain)
  • María Jesús del Hierro (CNB-CSIC, Madrid, Spain)
  • Marta Castrillo (CNB-CSIC, Madrid, Spain)
  • Isabel Martín-Dorado (CNB-CSIC, Madrid, Spain)
  • Kristy Kinchen (Mouse Biology Program, UC Davis, CA, USA)

Additional lectures by

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Alfonso Gutiérrez-Adán (INIA, Madrid, Spain)
  • Kent Lloyd (Mouse Biology Program, UC Davis, CA, USA)
  • Belén Pintado (CNB-CSIC, Madrid, Spain)
  • Jesús Martínez Palacio (CIEMAT, Madrid, Spain)
  • Javier Guillén (AAALAC, Pamplona, Spain)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)

The 2nd ISTT Young Investigator Award goes to Toru Takeo

Dr. Toru Takeo (Center for Animal Resources and Development, Kumamoto University, Japan). 2nd ISTT Young Investigator Award.
Dr. Toru Takeo (Center for Animal Resources and Development, Kumamoto University, Japan). 2nd ISTT Young Investigator Award.

The International Society for Trangenic Technologies (ISTT) is pleased to announce the second (2nd) ISTT Young Investigator Award, generously sponsored by inGenious Targeting Laboratory. The Award Committee carefully evaluated the excellent merits presented by all nominated candidates and decided to select Dr. Toru Takeo (Center for Animal Resources and Development, Kumamoto University, Japan) for the second ISTT Young Investigator Award. Dr. Toru Takeo was nominated by Dr. Jan Parker-Thornburg (ISTT Member, ISTT Council). The Award committee considered that Dr. Toru Takeo’s work represented a major improvement of mouse sperm cyropreservation and IVF techniques, thereby greatly facilitating the archiving and sharing of many mouse models produced by the transgenic community.

Dr. Toru Takeo, working in Dr. Naomi Nakagata’s laboratory, at CARD-KU, in Kumamoto (Japan), described three major technical improvements for boosting the efficiency of mouse sperm cryopreservation and the subsequently required IVF technique. The three fundamental modifications he reported in his recent publications tackled all three important steps in sperm cryopreservation and IVF, namely: (1) improving the cryopreservation of spermatozoa by adding L-Glutamine to the cryoprotective agent (CPA); (2) improving the number of capacitated sperm, upon thawing and pre-incubating them with Methyl-Beta-CycloDextrin (MBCD) by reducing the cholesterol levels in plasma membrane and increasing fertilization rate; and, (3) improving the in vitro fertilization (IVF) step itself, by adding reduced glutathione (GSH) to the IVF medium thereby increasing periviteline space in oocytes and the number of free thiols in the zona pellucida (ZP), greatly facilitating ZP penetration by sperm cells and, hence, fertilization. The three modifications, when jointly applied to some common mouse inbred strains, traditionally difficult to archive due to known poor sperm cryopreservation performance and limited IVF success, like C57BL/6J mice, boosted IVF efficiencies to >80%, and resulted in a true revolution in the transgenic mouse community and public archives. For example, at the European Mouse Mutant Archive (EMMA), a number of nodes have already updated their routine sperm cryopreservation and IVF methods according to Dr. Toru Takeo’s reports and succeeded in reproducing CARD innovations.

Dr. Toru Takeo’s three main publications on this subject are:

Takeo T, Nakagata N.
Reduced glutathione enhances fertility of frozen/thawed C57BL/6 mouse sperm after exposure to methyl-beta-cyclodextrin.
Biol Reprod. 2011 Nov;85(5):1066-72.

Takeo T, Nakagata N.
Combination medium of cryoprotective agents containing L-glutamine and methyl-{beta}-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse sperm.
Lab Anim. 2010 Apr;44(2):132-7.

Takeo T, Hoshii T, Kondo Y, Toyodome H, Arima H, Yamamura K, Irie T, Nakagata N.
Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells.
Biol Reprod. 2008 Mar;78(3):546-51.

Dr. Toru Takeo was born in 1979. In 2003, he obtained a Bachelor degree in Pharmaceutical Sciences at the University of Kumamoto and, subsequently, he continued his studies at the Graduate School of Pharmaceutical Sciences obtaining a Master degree in Pharmaceutical Sciences in 2005 and his PhD degree in Pharmaceutical Sciences in 2008. Thereafter, he has worked as postdoctoral researcher at the Division of Reproductive Engineeting, of the Center for Animal Resources and Development (CARD)-University of Kumamoto and, in 2010, he was promoted to Assistant Professor. He currently holds several professional memberships at various Societies, including the Japanese Association for Laboratory Animal Science, the Japan Pharmaceutical Association, The Pharmaceutical Society of Japan and the Society for Reproduction and Development, Japan, and his excellent work has already been awarded several times, including his most recent 2012 Young Investigator Award, by the Japanese Association for Laboratory Animal Science.

The 2012 ISTT Young Investigator Award Committee was formed by Dr. Lluis Montoliu (President of ISTT)-Chair, Dr. Thom Saunders (Vice-President of ISTT), Dr. Boris Jerchow (ISTT Council member), Dr. Paul Sheiffele (iTL CEO) and Dr. Lisa Aronov (iTL).

Dr. Toru Takeo will attend the next 11th Transgenic Technology (TT2013) meeting where he will receive the corresponding diploma and will deliver a short talk summarizing his scientific achievements that led him to receive this 2nd ISTT Young Investigator Award, sponsored by inGenious Targeting Laboratory (iTL).

Download this ISTT Press Release

the ISTT Young Investigator Award, sponsored by iTL
the ISTT Young Investigator Award, sponsored by iTL

	

Robert G. Edwards awarded the Nobel Prize of Medicine 2010 for the development of in vitro fertilization

Robert G. Edwards - Biographical. Nobelprize.org. 4 Oct 2010
"Robert G. Edwards - Biographical". Nobelprize.org. 4 Oct 2010

Robert G. Edwards (Manchester, UK, 1925), from the University of Cambridge, Cambridge, UK, has been awarded today, October 4, the 2010 Nobel Prize in Physiology or Medicine, “for the development of in vitro fertilization“. Robert G. Edwards and Patrick C. Steptoe (1913-1988) performed in 1977 the first successful in vitro fertilization (IVF) in humans, resulting in the birth of the first IVF baby, Louise Joy Brown, in 1978. Since then, 32 years later, more than 4 million children have been born thanks to this IVF technique. IVF was not only developed in humans, thanks to Robert G.Edward’ s pioneer experiments, started in the late 50’s, but in rodents too. Several scientists, including David Whittingham, managed to obtain mice by IVF in the early 70’s. IVF is a powerful technique for the treatment of human infertility and also a tool for producing synchronized embryos of high quality in many species, such as mice. IVF methods are routinely applied in many transgenic units for producing embryos that are suitable for cryopreservation. Subsequent technical developments in reproductive biology, such as ICSI (intracytoplasmic sperm injection), have enriched the toolbox available to gynecologists and scientists in general for obtaining viable mammalian embryos.