Videos from talks at the TT2013 meeting in China are available for ISTT members

Videos from talks at the TT2013 meeting in China are available for ISTT members
Videos from talks at the TT2013 meeting in China are available for ISTT members

We have uploaded videos from 25 of the 37 talks from selected invited speakers attending the past 11th Transgenic Technology meeting (TT2013), held in Guangzhou (China), on 25-27 February 2013, and organized by Ming Zhao and his co-worker Xiangguang Wu, from the Southern Medical University in Guangzhou. Ming Zhao and Xiangguang Wu kindly provided the original videos from those invited speakers that approved their distribution among ISTT members, from the members-only area of the ISTT web site. On behalf of the International Society for Transgenic Technologies (ISTT) I would like to thank both the speakers who approved the distribution of these video-talks and our colleagues from Guangzhou for technical help and for providing the original video files. The videos presented from TT2013 include the following talks:

Qi Zhou
The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)
From Haploid Stem Cells to Transgenic Mice

Jinsong Li
Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China
Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells

Naomi Nakagata
Center for Animal Resources and Development, Kumamoto University, Japan
Overview and Progress in sperm cryopreservation and IVF
10:30 – 11:15 Coffee Break, Posters and Exhibitors, 45 min break

Michael Dobbie
Australian Phenomics Facility, The Australian National University, Canberra, Australia
The Australian Phenomics Network: Creating and Delivering New Research Tools and Resources

Bo Zhang
College of Life Sciences, Peking University, Beijing, PR China
TALEN Mediated Genome Modification in Zebrafish

Liangxue Lai
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, PR China
Generation of PPAR? mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning

Benoît Kanzler
Max-Planck Inst. for Immunobiology and Epigenetics, Freiburg, Germany
Developing cutting edge technologies while still maintaining excellent basic services

Thomas Kolbe
Biomodels Austria & Inst. For Biotech. in Animal Production, Tulln, Austria
The business aspects of running a transgenic unit

Lluís Montoliu
National Center of Biotechnology, CSIC, Madrid, Spain; ISTT President
Introduction to the 9th ISTT Prize for outstanding contributions to transgenic technologies

Allan Bradley, 9th ISTT Prize
Wellcome Trust Sanger Institute, Hinxton, UK
Embryonic stem cell technology 1980-2013: A personal journey over three decades

Fernando Benavides
MD Anderson CC, Smithville, TX, USA
The impact of genetic background in mouse and rat models: concerns and solutions

Shannon Byers
The Jackson Laboratory, Bar Harbor, ME, USA
Assisted Reproduction Strategies in Mice Short Oral Presentations selected among submitted abstracts:

Francina Langa
Institut Pasteur, Paris, France
Frozen versus fresh embryos for ES cell microinjections: a comparative study with BALB/cN and C57BL/6N strains from seven transgenic facilities

Gonzalo Moreno
Instituto de Neurociencias de Alicante, CSIC/UMH, Alicante, Spain
A New Methology for serial sperm collection in Mice

Kyle D. Lutes
Department of Computer and Information Technology-CIT Faculty, Purdue University, West Lafayette, IN, USA
Computer software application options for laboratory data management

Malcolm France
Sydney, Australia
Animal ethics and animal rights: lessons from history and implications for the future

James Bussell
Wellcome Trust Sanger Institute, Hinxton, UKBalancing High Throughput Production and Phenotyping with Animal Welfare, Care and Husbandry

Wiebke Garrels
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Germany
Precision genetic engineering in the pig genome and skin transplantation between members of a syngenic clone cohort carrying different vital reporter transposon

Takashi Kohda
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Japan
Gene expression changes induced by intracitoplasmic sperm injection

Shoukhrat Mitalipov
Oregon National Primate Res. Center, OHSU, Beaverton, OR, USA
Primate chimeras and ES cells

Yann Herault
Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France
Overview of cre-mouse lines resources

Takashi Kuramoto
Institute of Laboratory Animals, Kyoto University, Kyoto, Japan
National BioResource Project for the Rat in Japan

Zhu-Gang Wang
Shanghai Research Center for Model Organisms, Shanghai, PR China
Studies on the physiological functions of novel G-protein-coupled receptors in mice

Pentao Liu
Wellcome Trust Sanger Institute, Hinxton, UK
Rapid and Efficient Reprogramming of Mouse Somatic Cells to iPS Cells Using Six Factors.

Toru Takeo, 2nd ISTT Young Investigator Award
Division of Reproductive Engineering, Center for Animal Resources and Development-CARD, Kumamoto University, Japan
Efficient production of mouse embryos via chemically assisted in vitro fertilization using frozen- thawed sperm

All these videos from talks at the TT2013 video are available for ISTT members, within the members-only area.

The TT2013 meeting and the ISTT at the Chinese science web portal biodiscover.com

The TT2013 meeting and the ISTT at the Chinese science web portal biodiscover.com
The TT2013 meeting and the ISTT at the Chinese science web portal biodiscover.com

The recent and most successful 11th Transgenic Technology (TT2013) meeting, held in Guagnzhou (China) on 25-27 February 2013 and organized by Prof. Ming Zhao (Southern Medical University)l, on behalf of the International Society for Transgenic Technologies (ISTT), has been reported, commented and covered by pictures, videos and TV- interviews at the most popular and visited Chinese science-oriented web portal biodiscover.com. Footage includes TV-interviews to Prof. Ming Zhao, Chair of the TT2013 meeting; Prof. Xiang Gao, Director of the Model Animal Research Center of Nanjing University, Nanjing, China; Prof. Zhu-gang Wang, Director of the Shanghai Research Center for Model Organisms, Shanghai, PR China; and Dr. Lluis Montoliu, President of the ISTT, among other.

Lluis Montoliu, President of the ISTT, interviewed at the TT2013 meeting for biodiscover.com
Lluis Montoliu, President of the ISTT, interviewed at the TT2013 meeting for biodiscover.com

NIH Course: TRAC 12:Transgenic Technology: Methods and Application, Jan 9-13, 2012

TRAC 12:Transgenic Technology: Methods and Application
TRAC 12:Transgenic Technology: Methods and Application

The TRAC 12:Transgenic Technology: Methods and Application Course is a four-day Biotechnology Training Courses at the National Institutes of Health that will be held on January 9-12, 2012. According to its web site: “This lecture/laboratory demonstration course is intended for those who wish to develop a strong background in transgenic techniques and applications. The two dominant approaches to transgenic technology will be emphasized, namely classic transgenic production by pronuclear microinjection and knockout transgenic production(gene targeting) by blastocyst injection with embryonic stem (ES) cells. The comprehensive coverage will address supporting technology (e.g. cryopreservation, rederivation, Intracytoplasmic Sperm Injection(ICSI), In Vitro Fertilization (IVF) , animal husbandry and surgery) as well as the molecular biology/recombinant DNA aspects of transgenesis.”

The TRAC12 Course will cover the following topics: Transgenic Technology overview (Embryonic Development, Optimizing Constructs for in vivo Expression, Preparation of Females for Embryo Collection, Pronuclear Microinjection, Generation of Pseudopregnant Females, Implantantation in Foster Mothers, Identification of Transgenic Progeny, Optimization of Breeding Population from Founders); Lentiviral and BAC transgenisis; Gene Targeted Transgenisis (Isolation and Maintenance of Totipotent embryonic Stem Cells for the Generation of Chimeric Mice; Homologous Recombination in ES Cells; Construct Design, Transfection, Selection; Generation of Gene Knockout Mice using Targeted ES Cells; Blastocyst Injection; Optimizing Germ-line Chimeras); Alternative Methods for Generating Targeted Mice: 8_cell Embryo Injection and ES Cell Aggregation; transgenic phenotype analysis; Transgenic Animal Model Supporting Techniques (Cryopreservation and rederivation; ICSI and IVF; Transgenic Model Future Prospects); Transgenic Model FuFuture Prospects; Sampling Project Question and Answer; Live and Video Demonstrations and hands on work (e.g. microinjection; Superovulation; Embryo Harvest and Transfer).

Applications to attend this TRAC 12 course can be submitted through this web site.

IVF and cryopreservation movies from CARD (Japan)

IVF and cryopreservation movies from CARD (Japan)
IVF and cryopreservation movies from CARD (Japan)

Members of Prof. Naomi Nakagata‘s laboratory, at the Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, Kumamoto, Japan, have uploaded various very interesting videos, publicly available from YouTube, illustrating several methods and techniques related to in vitro fertilization (IVF) and the cryopreservation of mouse lines, through sperm and embryos.

These are some of these video files uploaded in YouTube by CARD:

Classical videos on transgenic and KO mice from CSHL Press available now to ISTT members

Transgenic Techniques in Mice: A Video Guide by Roger A. Pedersen, Janet Rossant, © CSHL Press, 1989
Transgenic Techniques in Mice: A Video Guide by Roger A. Pedersen, Janet Rossant, © CSHL Press, 1989

The International Society for Transgenic Technologies is most proud to announce the release of the digital versions of the two classical video guides on transgenic and knockout mice, produced by Cold Spring Harbor Laboratory Press (CSHL Press) in 1989 and 1993, that will be now available to ISTT members for learning and/or teaching purposes, from the ISTT members-only restricted area, thanks to a most generous agreement reached with CSHL Press, owner of the copyright for both videos, marketed as analogic PAL/NTSC videos.

These two videos have been, for many years, unique and the most used and helpful video reference for anyone willing to start learning/teaching in the field of genetically modified mice. The first video is “Transgenic Techniques in Mice: A Video Guide“, by Roger A. Pedersen and Janet Rossant, produced by CSHL Press in 1989. The second video is “Targeted Mutagenesis in Mice: A Video Guide“, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, produced by CSHL Press in 1993.

Targeted Mutagenesis in Mice: A Video Guide, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, © CSHL Press, 1993
Targeted Mutagenesis in Mice: A Video Guide, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, © CSHL Press, 1993

The contents of the two videos are:

Transgenic Techniques in Mice: A Video Guide (CSHL Press, 1989)

  • Procedure 1: Dissection of oviducts. Recovery of fertilized eggs. Removal of cumulus cells.
  • Procedure 2: Recovery of 8-cell embryos. Removal of the zona pellucida. Construction of aggregation chimeras.
  • Procedure 3: Dissection of uteri. Recovery of blastocysts.
  • Procedure 4: DNA injection into pronuclei to produce transgenic embryos.
  • Procedure 5: Nuclear transfer.
  • Procedure 6: Blastocyst injection of embryonic stem cells.
  • Procedure 7: Vasectomizing male mice.
  • Procedure 8: Oviduct transfer of manipulated embryos.
  • Procedure 9: Uterine transfer of manipulated embryos.
  • Procedure 10: Recovery of 6-1/2 and 7-1/2 day embryos.
  • Procedure 11: Dissection of midgestation 12-1/2 day embryos and fetal membranes

Targeted Mutagenesis in Mice: A Video Guide (CSHL Press, 1993)

  • Demonstration 1: Starting embryonic stem (ES) cell lines
  • Demonstration 2: Making fibroblast feeder layers
  • Demonstration 3: Growing established ES cell lines
  • Demonstration 4: Targeting ES cell lines
  • Demonstration 5: Making chimeras with ES cells
  • Demonstration 6: Making a mouse