CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013
CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course that will be held at the National Centre for Biotechnology (CNB-CSIC), in Madrid, CSIC, during one week, on 7-11 October 2013, organized by Naomi Nakagata (CARD-Kumamoto University, Japan, Coordinator of CARD) and Lluis Montoliu (CNB-CSIC, Madrid, Spain, Coordinator of the Spanish EMMA node).

This course is open to anyone interested. Pre-application will be required, including, at least, a recent CV and a letter prepared by the intended participant describing how the applicant will benefit by attending this course and how relevant is the course material to his/her work. Additional documents are welcome, at the discretion of participants, including supporting letters by supervisors (where appropriate), reference letters, etc… Pre-applications should be submitted by email to cryocourse@cnb.csic.es by 31 May 2013.

The maximum number of participants attending this course will be 20, distributed among countries and institutions, and according the documentation provided and the interests expressed. Pre-applications will be accepted until 31 May 2013. The review and selection of participants will be done by the Organizers from 1 to 15 June 2013. Registrations and payments for selected participants will be accepted from June 16, 2013 to August 31, 2013. If required, an ordered waiting list will be prepared and any cancellation or unpaid registration by 31 August 2013 will be readily substituted by the first available person from this waiting list, starting on 1 September 2013.

The course registration fee is 800 Euros (with a reduced fee of 750 Euros for ISTT Members). This fee includes participation in the entire course, all materials and reagents, lunches over the 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, if required, at a convenient nearby hotel, close to CNB Campus, where all instructors and lecturers will be also lodged, hence further promoting interaction from breakfast to dinner. The official language of the course will be English.

COURSE INFORMATION: Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have boosted the mouse cryopreservation field with improved methods for fresh and frozen sperm techniques and associated optimized IVF methods that have resulted in unparalleled increased efficiencies for the cryopreservation and rescue of relevant mouse lines. At the CNB-CSIC in Madrid, hosting the Spanish EMMA node, these new CARD cryopreservation methods have been successfully implemented and, moreover, a fruitful collaboration has been established with the laboratory of Prof. Naomi Nakagata, eventually resulting in the signature of an institutional cooperation agreement between the CSIC and the University of Kumamoto, under the framework of which this cryopreservation course is organized. The aim of this course is to introduce the new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities and willing to implement these new methods, directly taught by the team which devised them. Each participant will have one stereomicroscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field. The number of instructors and lecturers appointed is 20.

Hands-on topics that will be covered during this cryopreservation course

  • making pipettes and practising embryo handling
  • isolating unfertilized mouse oocytes
  • isolating and cold storage/shipping of mouse cauda epididymis
  • freezing/thawing mouse sperm and IVF
  • fresh mouse sperm and IVF
  • freezing/thawing 2-cell IVF-derived mouse embryos
  • vitrification of mouse oocytes and embryos
  • embryo transfer techniques in mice
  • vasectomy of male mice
  • demonstration of ICSI

Additional lectures on the following topics

  • new EU Directive on the protection of animals for experimentation
  • new US Guidelines for the use of animals in research
  • epigenetic effects of in vitro culture on mouse embryos
  • databases for handling information in cryopreservation facilities
  • shipping mice, refrigerated and frozen material
  • safety issues handling liquid nitrogen in a cryopreservation facility
  • EMMA and Infrafrontier-I3
  • MMRRC

Instructors

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Kiyoko Fukumoto (CARD-Kumamoto University, Japan)
  • Tomoko Kondo (CARD-Kumamoto University, Japan)
  • Yukie Haruguchi (CARD-Kumamoto University, Japan)
  • Yumi Takeshita (CARD-Kumamoto University, Japan)
  • Yuko Nakamuta (CARD-Kumamoto University, Japan)
  • Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Raul Fernández (INIA, Madrid, Spain)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)
  • Julia Fernández (CNB-CSIC, Madrid, Spain)
  • María Jesús del Hierro (CNB-CSIC, Madrid, Spain)
  • Marta Castrillo (CNB-CSIC, Madrid, Spain)
  • Isabel Martín-Dorado (CNB-CSIC, Madrid, Spain)
  • Kristy Kinchen (Mouse Biology Program, UC Davis, CA, USA)

Additional lectures by

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Alfonso Gutiérrez-Adán (INIA, Madrid, Spain)
  • Kent Lloyd (Mouse Biology Program, UC Davis, CA, USA)
  • Belén Pintado (CNB-CSIC, Madrid, Spain)
  • Jesús Martínez Palacio (CIEMAT, Madrid, Spain)
  • Javier Guillén (AAALAC, Pamplona, Spain)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)

IX Transgenic Animal Research Conference, Tahoe City, CA, USA, 11-15 August 2013

IX Transgenic Animal Research Conference, Tahoe City, CA, USA, 11-15 August 2013
IX Transgenic Animal Research Conference, Tahoe City, CA, USA, 11-15 August 2013

The International Society for Transgenic Technologies (ISTT) has decided, once again, to co-sponsor the IX Transgenic Animal Research Conference, hosted by the Department of Animal Science, UC Davis, and organized by ISTT Member Prof. James D. Murray. This conference will be held at the Granlibakken Conference Center, in Tahoe City, CA, USA, on 11-15 August 2013. This is a classical conference, most complementary to the TT meeting series, and specifically devoted to basic and applied projects, research and technical developments using non-rodent genetically modified animals. The ISTT is proud to have supported the two previous UC Davis Transgenic Conferences in Tahoe held in 2009 and 2011.

According to the conference web site: This is the ninth international meeting hosted by UC Davis to bring together representatives from the leading laboratories worldwide doing cutting edge work on transgenic research in non-murine animals, including livestock, fish and poultry species. The previous meetings were each attended by up to 160 participants from 12 to16 different countries throughout the globe. Each conference, in addition to reviews and papers on transgenic animals, included presentations covering technical developments in areas such as nuclear transfer-based cloning, cell transformation, vector design, and nuclease-directed gene insertion that affect the production of transgenic animals. Oral presentations are by invitation, with participants encouraged to contribute poster presentations. The upcoming conference will again focus on state-of-the-art science in the field of transgenic research. Presentations will address cutting-edge methodology, technical improvements, and current progress towards producing transgenic animals for biomedical and agricultural applications. The intent of these meetings is to bring together scientists to discuss progress, problems, and potential application of transgenic technology for animal applications. The meeting will consist of invited presentations and submitted posters. Two afternoons from noon to 4 p.m. and one evening will be free to allow for small group interactions and to take advantage of the great natural beauty and recreational activities in the Lake Tahoe area.

Already appointed and confirmed Speakers include:

  • Matt Wheeler (Illinois) – opening talk
  • Bruce Whitelaw (Edinburgh) – closing talk , ISTT Member
  • Scott Fahrenkrug (Minnesota) – TALENS in livestock
  • Emmanuelle Charpentier (Germany/Sweden) – CRISPR RNA-programming technology
  • Caitlin Cooper (Davis) – feeding hLZ and hLF milk to pigs
  • Hongbin He (China) – FMDV resistance
  • Angelika Schnieke (Germany) – cancer models in pigs
  • Chuck Long (A&M) lentiviral production of livestock
  • Irina Polejaeva (Utah) – tg goat cardiovascular models
  • Goetz Laible or Stefan Wagner (NZ) – KO of beta-lactoglobulin and transduction of the mammary gland
  • Rob Etches (Crystal Biosciences) – heavy chain KO chickens
  • Yonglun Luo (Alun) (Denmark) – adeno-assoc HR + talens / BCRA-1
  • Mike McGrew (Roslin) growing and modifying avian primordial germ cells, including transposons
  • Tim Doran (Aust) Virus disease resistant transgenic poultry and fish
  • Derek Nimmo (UK) – TG mosquito for control of dengue fever
The complete registration packet for this IX TARC conference includes: conference registration fee, four night’s accommodation at Granlibakken, all meals, refreshments, receptions and ground transportation from Davis or Reno (if needed) (USD 1,600). ISTT Members are entitled to a reduced registration fee (USD 1,550). Registrations received after June 14, 2012 are NOT guaranteed.

7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013
7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the 7th Workshop on Innovative Mouse Models (IMM2013), which will be held at the Leiden University Medical Center (LUMC), in Leiden, The Netherlands, on 13-14 June 2013. This will be the third consecutive edition of this meeting series that is granted with the co-sponsorship of the ISTT, after the IMM2011 and the IMM2009 editions, due to its interest and relevance for the community of researchers using genetically-modified mice. The IMM2013 workshop is organized by: Jos Jonkers (NKI-AVL, Amsterdam, The Netherlands), Paul Krimpenfort (NKI-AVL, Amsterdam, The Netherlands), Werner Mueller (University of Manchester, Manchester, United Kingdom), Hein te Riele (NKI-AVL, Amsterdam, The Netherlands), Els Robanus-Maandag (LUMC, Leiden, The Netherlands), Marian van Roon (VU, Amsterdam, The Netherlands and ISTT Member) and Sjef Verbeek (LUMC, Leiden, The Netherlands and ISTT Member).

According to the IMM2013 workshop web page: The primary goal of this two-day workshop is to bring together a diverse group of scientists interested in advanced genome alteration approaches in the mouse, including key developers of emerging technologies as well as researchers who wish to apply and assess these new approaches. The IMM2013 workshop will encourage an in-depth and unvarnished discussion of these technologies and novel developments. This 2-day workshop will have a mixture of invited speakers and selected presentations. Keynote lectures will be given by:

  • Anton Wutz (UK) haploid ESC
  • Haoyi Wang (US) iPS / TALENs
  • Bill Skarnes (UK) high throughput TALENs
  • Ben Davis (UK) docking site/ new recombinases
  • Yann Herault (France) Cre transgenic mice
  • Kevin Brindle (UK) MRI
  • Mathijs Verhagen (NL) large scale phenotyping
  • B Kappes (US) TALENs
  • Zoltan Ivics (Germany) Transposons

TT2013 meeting in China coming soon!

TT2013 meeting in China coming soon!
TT2013 meeting in China coming soon!

Dear colleagues,

In about two weeks, and starting on February 25, the International Society for Transgenic Technologies (ISTT) will be holding the 11th Transgenic Technology (TT2013) meeting, at the Baiyun International Convention Center, in Guangzhou/Canton, Guandong, China, followed by the hands-on workshop on basic microinjection techniques. The TT2013 meeting is the most important event promoted from the International Society for Transgenic Technologies (ISTT). The TT2013 meeting is organized by Prof. Ming Zhao (China) and the subsequent hands-on workshop by Dr. Wenhao Xu (USA), Prof. Liangping Li (China) and Prof. Ming Zhao.

The official TT2013 meeting web site with all the relevant information for this ISTT conference is available from www.tt2013.org or www.tt2013.com

The TT2013 meeting web page within the ISTT web site is available from: www.transtechsociety.org/tt2013/

I would like to thank all Organizer and Advisory Committees, all Exhibitors and Sponsors for contributing with their work and support to the success of the TT2013 meeting.

See you soon in China!

Lluis