Course on Genetic Manipulation of ES Cells, Hinxton, Cambridge, UK, 5-18 November 2012

Course on Genetic Manipulation of ES Cells
Course on Genetic Manipulation of ES Cells

Course on Genetic Manipulation of ES Cells, 5-18 November 2012
Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Deadline for applications: 6 July 2012

Course summary
This laboratory-based training course will provide a comprehensive overview and practical laboratory experience of the genetic manipulation of mouse ES cells for a broad range of applications. Recent advances in genome informatics, recombineering, transposon technology and uses of conditional gene targeting will be covered in lectures by both instructors and invited expert speakers and through interactive demonstrations.Laboratory work will focus on the culture and transfection of ES cells, design and construction of gene targeting vectors from BACs by recombineering, genotyping of gene targeting events and the practical use of transposon technology. Participants will also be trained in the informatics and practical use of public gene targeting resources being produced by the IKMC (International Knockout Mouse Consortium).

Course instructors
Professor Francis Stewart (Dresden University of Technology, Dresden, Germany)
Dr William Skarnes (Wellcome Trust Sanger Institute, Cambridge, UK)
Dr Pentao Liu (Wellcome Trust Sanger Institute, Cambridge, UK)
Dr Barry Rosen (Wellcome Trust Sanger Institute, Cambridge, UK)

The Course programme includes the following topics:

1. Informatics
The informatics underlying the visualization of gene structures and the design of gene targeting vectors, recombineering oligos and genotyping primers will be demonstrated. Students will also run their own gene targeting designs using web-based tools.The informatics of locating existing public IKMC gene targeting resources will also be covered.

2. Recombineering of Gene Targeting Vectors
Students will build a variety of targeting constructs from BACs using recombineering technology. The theoretical principles underlying both recombineering and rational targeting vector design will be emphasized by both lectures and practical exercises.

3. ES Cell Culture
Students will learn feeder-dependent and feeder-free culture of ES cells derived from 129 and C57BL/6 mouse strains.ES cell colonies will be picked, expanded and frozen and subsequently thawed to test their integrity.

4. Gene Targeting in ES Cells
Students will electroporate conditional gene targeting constructs into ES cells and learn to genotype cells by LR-PCR and qPCR-based methods.

5. Transposon Technology
Students will be introduced to the uses of the highly efficient piggyBac transposon system for expression, mutagenesis and mouse induced pluripotent stem cell (iPS) cell generation.

6. Modular Targeting Resources
Students will assemble a variety of modular knock-in targeting vectors from IKMC resources and analyse their integrity.Recombination Mediated Cassette Exchange (RMCE) using Flp and Cre recombinases will be used to modify IKMC conditional alleles directly in ES cells.

Additional information and instructions to apply are available from the web site of this course

ISTT will be present at the CALAS Symposium

ISTT will be present at the CALAS Symposium
ISTT will be present at the CALAS Symposium

At the International Society for Transgenic Technologies (ISTT), we are pleased to announce our participation as CALAS Affiliate exhibitor in the next (51st) CALAS Symposium, to be held in Vancouver, on June 2-5, 2012. CALAS is the Canadian Association for Laboratory Animal Science (or ACSAL, Association Canadienne pour la Science des Animaux de Laboratoire). The CALAS aims and mission are if full agreement with our ISTT bylaws, therefore at the ISTT we are most happy to collaborate and explore further interactions with CALAS, as we have done, successfully, with other similar societies in the field.

According to the CALAS web site, “CALAS has been working since 1961 to eliminate inhumane and unnecessary use of animals in research. Working with a membership comprised of veterinarians, physicians, researchers, administrators and technicians, CALAS has achieved many successes in improving the care of laboratory animals in research, teaching and testing. CALAS is a not-for-profit organization with approximately 1000 members, and strives to advance the knowledge, skills and status of those who work with laboratory animals; to improve the standards for laboratory animal care and research, and; to provide a forum for the exchange and dissemination of knowledge regarding animal care and research”.