The second edition of the International Course on “Genetics of Laboratory Rodents” was held at the Institut Pasteur-Montevideo, Uruguay, from December 5 to 14, 2011, with the co-sponsorization of the International Society for Transgenic Technologies (ISTT). This course was organized by Martina Crispo, DVM – Head of the Transgenic and Experimental Animal Unit – Institut Pasteur de Montevideo, Uruguay, and ISTT Member, and co-organized by Jean Jacques Panthier, PhD – Unité de Genetique Fonctionelle de la Souris – Institut Pasteur, France. The teaching team was composed by Scientists from the Institut Pasteur in Paris and Montevideo as well as other regional and international institutions, who are familiar with the topics, including several ISTT Members (Jean Jaubert, Fernando Benavides, Ignacio Anegon). Students (20 participants, coming from Argentina, Chile, Brazil, Netherlands and Uruguay) were carefully selected among a long list of candidates. Again, as in its first edition, organized in 2008, this course was a very successful event, both from the scientific and social points of view, allowing more than 30 researchers from different countries to spent together ten days for science discussion. Furthermore, all students received credits for their curricula, meaning that this course will be considered as a significant element of their MSc or PhD programs. A full scientific course report is available from the ISTT web site. The ISTT wishes to thank Martina Crispo and her team from the Institut Pasteur de Montevideo, along with the rest of organizers and teachers participating, for succesfully running the second edition of this educational initiative on Laboratory Rodents Genetics in South-America.