The second edition of the International Course on “Genetics of Laboratory Rodents” was held at the Institut Pasteur-Montevideo, Uruguay, from December 5 to 14, 2011, with the co-sponsorization of the International Society for Transgenic Technologies (ISTT). This course was organized by Martina Crispo, DVM – Head of the Transgenic and Experimental Animal Unit – Institut Pasteur de Montevideo, Uruguay, and ISTT Member, and co-organized by Jean Jacques Panthier, PhD – Unité de Genetique Fonctionelle de la Souris – Institut Pasteur, France. The teaching team was composed by Scientists from the Institut Pasteur in Paris and Montevideo as well as other regional and international institutions, who are familiar with the topics, including several ISTT Members (Jean Jaubert, Fernando Benavides, Ignacio Anegon). Students (20 participants, coming from Argentina, Chile, Brazil, Netherlands and Uruguay) were carefully selected among a long list of candidates. Again, as in its first edition, organized in 2008, this course was a very successful event, both from the scientific and social points of view, allowing more than 30 researchers from different countries to spent together ten days for science discussion. Furthermore, all students received credits for their curricula, meaning that this course will be considered as a significant element of their MSc or PhD programs. A full scientific course report is available from the ISTT web site. The ISTT wishes to thank Martina Crispo and her team from the Institut Pasteur de Montevideo, along with the rest of organizers and teachers participating, for succesfully running the second edition of this educational initiative on Laboratory Rodents Genetics in South-America.
The International Society for Transgenic Technologies (ISTT) wishes all of you Merry Christmas and a Happy New Year!. The 2011 ISTT Christmas Postcard prepared this year uses a wonderful chimeric mouse picture, obtained by Benoît Kanzler (Freiburg, Germany), almost brought back to life, while Seasonal Greetings are displayed using most of the languages currently used in the 40 countries of origin where our ISTT members work, world-wide.
2011 has been a fantastic year for the ISTT where many projects, initiatives and meetings have been successfully completed. Just to name a few: we published with Springer our first ISTT Manual on “Advanced Protocols for Animal Transgenesis“, edited by Shirley Pease and Thom Saunders; we were approved as a new AALAC’s member organization; we enjoyed a fantastic TT2011 meeting in Florida in October; we prepared a 10th TT Meetings Anniversary booklet; and, finally, and most importantly, we succeeded in moving the historical transgenic-list (tg-l) to the ISTT web server. We ended up 2011 with 573 members registered which we hope will renew their memberships in 2012, and where yet another list of interesting initiatives around the generation and analysis of transgenic animals will be prepared or supported from the ISTT. In 2012 there will be no TT meeting. Next TT meeting has been already scheduled for 2013. The TT2013 meeting will be held in Guangzhou/Canton, PR China, on February 25-27, 2013, organized by Prof. Ming Zhao (SMU).
Today, December 21st, 2011, the historical transgenic-list (tg-l), created by Peter Sobieszczuk more than 15 years ago, in July, 1996, has been moved and successfully transferred to its new home at the ISTT web server, following the agreement signed on September 30th, 2011, between Peter and the ISTT. All current subscribers have been transferred automatically and they will be able to continue using and enjoying this useful resource within the field of transgenic animals. This is the new tg-l home web page, where additional information about this transfer and the new features of the tg-l at the ISTT can be browsed. At the ISTT, the tg-l will continue being public (anyone may subscribe, including ISTT members and non-members alike, but subject to email address verification), unmoderated (messages will not be altered by the list administrator) and closed (only subscribers may post messages). All questions, comments, requests, doubts should be addressed to the new tg-l administrators at: firstname.lastname@example.org.
The TRAC 12:Transgenic Technology: Methods and Application Course is a four-day Biotechnology Training Courses at the National Institutes of Health that will be held on January 9-12, 2012. According to its web site: “This lecture/laboratory demonstration course is intended for those who wish to develop a strong background in transgenic techniques and applications. The two dominant approaches to transgenic technology will be emphasized, namely classic transgenic production by pronuclear microinjection and knockout transgenic production(gene targeting) by blastocyst injection with embryonic stem (ES) cells. The comprehensive coverage will address supporting technology (e.g. cryopreservation, rederivation, Intracytoplasmic Sperm Injection(ICSI), In Vitro Fertilization (IVF) , animal husbandry and surgery) as well as the molecular biology/recombinant DNA aspects of transgenesis.”
The TRAC12 Course will cover the following topics: Transgenic Technology overview (Embryonic Development, Optimizing Constructs for in vivo Expression, Preparation of Females for Embryo Collection, Pronuclear Microinjection, Generation of Pseudopregnant Females, Implantantation in Foster Mothers, Identification of Transgenic Progeny, Optimization of Breeding Population from Founders); Lentiviral and BAC transgenisis; Gene Targeted Transgenisis (Isolation and Maintenance of Totipotent embryonic Stem Cells for the Generation of Chimeric Mice; Homologous Recombination in ES Cells; Construct Design, Transfection, Selection; Generation of Gene Knockout Mice using Targeted ES Cells; Blastocyst Injection; Optimizing Germ-line Chimeras); Alternative Methods for Generating Targeted Mice: 8_cell Embryo Injection and ES Cell Aggregation; transgenic phenotype analysis; Transgenic Animal Model Supporting Techniques (Cryopreservation and rederivation; ICSI and IVF; Transgenic Model Future Prospects); Transgenic Model FuFuture Prospects; Sampling Project Question and Answer; Live and Video Demonstrations and hands on work (e.g. microinjection; Superovulation; Embryo Harvest and Transfer).