Members of Prof. Naomi Nakagata‘s laboratory, at the Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, Kumamoto, Japan, have uploaded various very interesting videos, publicly available from YouTube, illustrating several methods and techniques related to in vitro fertilization (IVF) and the cryopreservation of mouse lines, through sperm and embryos.
These are some of these video files uploaded in YouTube by CARD:
- Collection of Oocytes
- remove-ce [remove cauda epididymides]
- Preparation of sperm suspension
- Insemination by frozen-thawed sperm (IVF)
- Warm straw which includes frozen sperm suspension of mice
- Transferring thawed sperm suspension into a drop of FERTIUP
- Float straws including mice sperm suspension and freeze it
- Thawing of vitrified mice embryos
- Thawing of vitrified mice embryos-recommemded speed of pipetting-
- Dryshipper (in Japanese)
The YouTube link to the movie about “remove-ce” has been updated to its English version, thanks to people at CARD, who kindly uploaded the translated version of this interesting movie file.