Classical videos on transgenic and KO mice from CSHL Press available now to ISTT members

Transgenic Techniques in Mice: A Video Guide by Roger A. Pedersen, Janet Rossant, © CSHL Press, 1989
Transgenic Techniques in Mice: A Video Guide by Roger A. Pedersen, Janet Rossant, © CSHL Press, 1989

The International Society for Transgenic Technologies is most proud to announce the release of the digital versions of the two classical video guides on transgenic and knockout mice, produced by Cold Spring Harbor Laboratory Press (CSHL Press) in 1989 and 1993, that will be now available to ISTT members for learning and/or teaching purposes, from the ISTT members-only restricted area, thanks to a most generous agreement reached with CSHL Press, owner of the copyright for both videos, marketed as analogic PAL/NTSC videos.

These two videos have been, for many years, unique and the most used and helpful video reference for anyone willing to start learning/teaching in the field of genetically modified mice. The first video is “Transgenic Techniques in Mice: A Video Guide“, by Roger A. Pedersen and Janet Rossant, produced by CSHL Press in 1989. The second video is “Targeted Mutagenesis in Mice: A Video Guide“, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, produced by CSHL Press in 1993.

Targeted Mutagenesis in Mice: A Video Guide, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, © CSHL Press, 1993
Targeted Mutagenesis in Mice: A Video Guide, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, © CSHL Press, 1993

The contents of the two videos are:

Transgenic Techniques in Mice: A Video Guide (CSHL Press, 1989)

  • Procedure 1: Dissection of oviducts. Recovery of fertilized eggs. Removal of cumulus cells.
  • Procedure 2: Recovery of 8-cell embryos. Removal of the zona pellucida. Construction of aggregation chimeras.
  • Procedure 3: Dissection of uteri. Recovery of blastocysts.
  • Procedure 4: DNA injection into pronuclei to produce transgenic embryos.
  • Procedure 5: Nuclear transfer.
  • Procedure 6: Blastocyst injection of embryonic stem cells.
  • Procedure 7: Vasectomizing male mice.
  • Procedure 8: Oviduct transfer of manipulated embryos.
  • Procedure 9: Uterine transfer of manipulated embryos.
  • Procedure 10: Recovery of 6-1/2 and 7-1/2 day embryos.
  • Procedure 11: Dissection of midgestation 12-1/2 day embryos and fetal membranes

Targeted Mutagenesis in Mice: A Video Guide (CSHL Press, 1993)

  • Demonstration 1: Starting embryonic stem (ES) cell lines
  • Demonstration 2: Making fibroblast feeder layers
  • Demonstration 3: Growing established ES cell lines
  • Demonstration 4: Targeting ES cell lines
  • Demonstration 5: Making chimeras with ES cells
  • Demonstration 6: Making a mouse

Course on Laboratory Animal Science at the BSRC Alexander Fleming Institute, Vari, Greece

Course on Laboratory Animal Science at the BSRC Alexander Fleming Institute, Vari, Greece
Course on Laboratory Animal Science at the BSRC Alexander Fleming Institute, Vari, Greece

BSRC Alexander Fleming, with a longstanding reputation of excellence across Europe and a wealth of experience in the field of animal sciences, organizes in a yearly basis since the year 2007, the International two-week intensive Course on Laboratory Animal Science, the first such course to be held in Greece. The 2011 edition will be held on September 19-30. Here you can download a brochure for this course.
This prestigious event is addressed to professionals from fields of Biomedical Science. Past participants include biologists, veterinarians, medical practitioners and pharmacologists from all across Europe, Asia and Africa. This is a revolutionary course within the scientific community as it awakes a new wave of consciousness regarding animal welfare and quality research. The vision is to train a new breed of scientists with acute awareness of what humane research actually means. It breaks with tradition in the fact that it trains participants not only in the passive acquisition of knowledge, but in the active process of critical thinking. The fundamental principle the course aims to promote is that the experimental outcome of research strongly depends on the humane treatment of the laboratory animals.
Upon the completion each participant is able to understand and perform humane use and care of laboratory animals, and clearly comprehend the vital principles for quality research. Successful participants are awarded a certificate, now a requirement according to the revised European Directive, which demonstrates competence in working with laboratory animals for research purposes.
Theoretical part subjects:
• Biology and husbandry of laboratory animals
• Microbiology
• Health hazards and safe practices in the animal house
• Design and conduct of animal experiments
• Experimental Data Statistical analysis
• Ethical aspects and legislation
• Cost Benefit analysis
• Alternatives to animal use
• Euthanasia – humane methods
• Anaesthesia analgesia and experimental procedures
• Analysis of scientific literature
• Nutrition
• Genetic standardization
• The principles of transgenic/ knockout animals
• ENU mutagenesis
• Cloning-embryonic stem cells
• Cryo-preservation
• FELASA health screening-recommendations
• infectious and non infectious diseases of rats and mice
• Breeding laboratory animals
• Guidelines for safe transportation of laboratory animals
• Phenotype relative to environment
• Zoonoses
Practical part subjects:
• demonstration of animal handling and experimental techniques used in animal research (anaesthesia, necropsy)
• analysis of research articles; team exploration of a scientific text to establish particular strengths and weaknesses
• research protocol creation and presentation to an in-house ethics committee

Short list of collaborating lecturers:
Paul Flecknell Prof., Director Comparative Biology Centre, The Medical School, University of Newcastle; Courses 2009 , 2010
Baumans Vera, Dr. DVM, PhD, DipECLAM, Laboratory Animal Science Specialist. Professor, Dpt of Animals in Science & Society, Div Laboratory Animal Science, Ultrecht University, Holland; Courses 2007, 2008, 2009, 2010
Kathleen Pritchett-Corning Dr., DVM, DACLAM, MRCVS. Director, European Scientific Services Charles River Laboratories, UK; Course 2009
Cory Brayton Prof., DVM, Diplomate, ACLAM, Diplomate ACVP. Associate Professor of Molecular and Comparative Pathobiology.Director, Phenotyping Core, Johns Hopkins University, MD, USA; Courses 2009, 2010
Michael Festing, Ph.D., D.Sc., C.Stat., member of UK’s Animal Procedures Committee, trustee of the Fund for the Replacement of Animals in Medical Experiments (FRAME); Course 2011
Wallace James Dr., PhD. Editor, Science Associates, ALN Magazine, UK; Course 2009
Dedicated Site and newsgroup:

To support the diffusion of knowledge across past participants, lecturers of the LAS course and selected scientists, a dedicated Site and newsgroup have been created.
The site where the latest details on upcoming course (registration, program), pre-teaching material and videos of lectures of past courses (for selected registered members only) can be found at www.las-course.eu
The newsgroup is accessible at https://groups.google.com/group/las-course . Currently the newsgroup services more than 80 members.
For further information related to the course and newsgroup please refer to kornarou {at} fleming.gr

TT2011 meeting: updated information

TT2011 meeting: invited speakers
TT2011 meeting: invited speakers

An updated scientific and social program is available for the 10th Transgenic Technology Meeting, TT2011, to be held at St Pete Beach, Florida, on October 24-26, 2011. Additional information about the invited speakers is also available from the TT2011 meeting web site.

IMPORTANT DEADLINES:

Abstracts for the TT2011 meeting can be sent up to June 30.

Applications for ISTT Registration Awards, sponsored by the ISTT, and ISTT Young Investigator Award, sponsored by InGenious Targeting Laboratory, can be filed up to June 30.

Reduced fee registrations for the TT2011 meeting will be available up to July 31.

Advanced Protocols for Animal Transgenesis. An ISTT Manual (Due: August 2011)

Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)
Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

The International Society for Transgenic Technologies (ISTT), in collaboration with Springer, is pleased to announce the next publication of the book entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thomas L. Saunders, whose 1st edition is expected to be published by August 2011.

This laboratory manual, published by Springer in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories, most of them members of the ISTT. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Table of contents:

  • Karen S. Canady: Patent and licensing issues in transgenic technology.
  • Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.
  • Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.
  • Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.
  • Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.
  • Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.
  • Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal , Anne-Laure Iscache and Ignacio Anegon: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.
  • Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.
  • Michael G. Zeidler, Margaret L. Van Keuren and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.
  • Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.
  • Aron Geurts, Lajos Mates and Darius Balciunas: Vertebrate Transgenesis by Transposition.
  • Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson and F. Kent Hamra: Rat Spermatogonial Stem Cell Mediated Gene Transfer.
  • Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.
  • Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.
  • Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.
  • Marina Gertsenstein: Tetraploid Complementation Assay.
  • Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara and Marina Gertsenstein: Combining ES cells with Embryos.
  • Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.
  • Ping Li, Eric N Schulze, Chang Tong and Qi-Long Ying: Rat Embryonic Stem Cell Derivation and Propagation.
  • Han Li, Katerina Strati, Verónica Domínguez, Javier Martín, María Blasco, Manuel Serrano and Sagrario Ortega: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.
  • Anna B. Auerbach, Peter J. Romanienko and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.
  • Karen Brennan: Colony Management.
  • Belen Pintado and Juan Hourcade: Cryopreservation.
  • Shirley Pease: Shipment of Mice and Embryos.
  • Jorge M. Sztein, R.J. Kastenmayer and K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.
  • Jan Parker-Thornburg: Refinement, Reduction and Replacement

ISTT Members are entitled to a 33% discount on the book price.

ISTT co-sponsors the 6th Workshop on Innovative Mouse Models (IMM2011)

6th Workshop on Innovative Mouse Models (IMM2011)
6th Workshop on Innovative Mouse Models (IMM2011)

The International Society for Transgenic Technologies (ISTT) is pleased to co-sponsor the 6th Workshop on Innovative Mouse Models (IMM2011), to be held at the Leiden University Medical Center (LUMC), Leiden, The Netherlands, on May 12-13, 2011. The IMM2011 workshop is organized by Marian van Roon (VU, Amsterdam), Sjef Verbeek (LUMC), both ISTT members, along with Jos Jonkers, Paul Krimpenfort, Werner Mueller, Hein te Riele and Els Robanus-Maandag. Invited speakers include: Tomas Cermak, Ivo Huijbers, Rob Howes, Samer Hussein, Ralf Kühn, Guillermo Montoya and Francis Stewart. Deadline for registration is Tuesday April 26. ISTT members are entitled to a reduced registration fee. Please, check IMM2011 workshop registration web page for more detailed information.

The aim of this two-day workshop is to bring together a diverse group of scientists interested in advanced genome alteration approaches in the mouse, including key developers of emerging technologies as well as researchers who wish to apply and assess these new approaches. The ISTT is most happy to support, once again, the IMM2011 workshop, after having already co-sponsored the previous and most successful edition of this workshop series, the IMM2009.

Renewed agreement signed between ISTT and Springer

The International Society for Transgenic Technologies (ISTT) is associated with the scientific journal Transgenic Research, published by Springer
The International Society for Transgenic Technologies (ISTT) is associated with the scientific journal Transgenic Research, published by Springer

The International Society for Transgenic Technologies (ISTT) is pleased to announce that a renewed cooperation agreement has been signed with Springer, Publisher of the scientific journal “Transgenic Research“, associated to ISTT. Through this agreement, ISTT members will continue to benefit from a free online access to Transgenic Research full contents through the members-only area within the ISTT web site. Furthermore, ISTT Members will be entitled to a 33% discount on Springer books related to the field of animal genetics or animal transgenesis.

Please visit the JOIN web page to apply for ISTT membership in order to qualify for full free online access to Transgenic Research contents through the members-only area of the ISTT web site.