A manuscript entitled “Genetic polymorphisms among C57BL/6 mouse inbred strains” has been just published by Esther Zurita, Mónica Chagoyen, Marta Cantero, Rosario Alonso, Anna González-Neira, Alejandro López-Jiménez, José Antonio López-Moreno, Carlisle P. Landel, Javier Benítez, Florencio Pazos and Lluís Montoliu in Transgenic Research. The Illumina® Mouse Medium Density Linkage Mapping panel, with 1,449 single nucleotide polymorphisms (SNPs), was used to genotype individuals from ten C57BL/6-related strains: C57BL/6JArc, C57BL/6J from The Jackson Lab, C57BL/6J from Crl, C57BL6/JRccHsd, C57BL/6JOlaHsd, C57BL/6JBomTac, B6(Cg)-Tyr (<c-2j>)/J, C57BL/6NCrl, C57BL/6NHsd and C57BL/6NTac. Twelve SNPs were found informative to discriminate between the “/N” and “/J” C57BL/6 mouse substrains considered. These results will be instrumental for the correct genetic monitoring and appropriate mouse colony handling of different transgenic and knockout mice produced in distinct C57BL/6 inbred substrains. The SNP raw data are also available from the Mouse Phenome Database at Jackson Laboratory.
The International Society for Transgenic Technologies (ISTT) web site includes a list of transgenic laboratories and facilities located around the world. In addition, the web page now features a Google Maps script that indicates the geographical location of each of the transgenic facilities listed. If your facility is not yet included in this web page, please contact us (email: firstname.lastname@example.org) and we will include it right away. Thanks for your collaboration.
The Istituto di Biologia Cellulare of Consiglio Nazionale delle Ricerche-“A. Buzzati-Traverso” Campus and The Jackson Laboratory offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries. This Course is financially supported by the EU FP7 Capacities Specific Program, through the European Mouse Mutant Archive (EMMA). The course will be held at the “A. Buzzatti-Traverso” Campus, in Monterotondo, Rome, Italy on October 25-29, 2010.
The course is offered to teach methods in cryopreservation for banking of various strains of mice used in research, including inbred, transgenic and knock-out strains. Several methods of cryopreservation are now available and because no single method is adequate for all the various strains of mice being developed, a variety of methods are taught. The course is designed primarily as a “hands-on” laboratory program in which participants learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries.
Deadline for submitting applications: September 25, 2010