The most updated scientific program of this conference includes presentations on a variety of issues related to animal transgenesis, animal welfare, biotechnology, use of transposons in transgenesis, use of zinc-finger nuclease strategies in transgenesis, ES and iPS cells in severals species, biosafety issues, risk analysis and environmental risk assessment, antibody production, protein production in the milk of transgenic animals, RNAi and amny additional topics. It will also include a US FDA/CVM workshop on the regulation of recombinant DNA constructs in genetically modified animals.
Science magazine publishes tody a report by Geurts et al. describing the first successful attempt to produce gene targeted knockout rats through the use of Zinc Finger Nuclease technology, developed by Sangamo BioSciences, Inc. This strategy does not require the use of embryonic stem (ES) cells. Targeted mutations are induced by standard microinjection of DNA and RNA molecules into 1-cell rat embryos. This milestone has been achieved by a collaborative effort of academic and industrial scientists coordinated by the biotechnology company Open Monoclonal Technology, Inc. The team includes two members of ISTT, Ignacio Anegon and Séverine Ménoret, from the Transgenic Rats Common Facility at INSERM, UMR 643, CHU, Nantes, Université de Nantes, France. Ignacio Anegon has confirmed his participation, as invited speaker, at the forthcoming TT2010 meeting, to be held in Berlin, March 22-24, 2010.
In this work, OMT scientists and their collaborators have made use of zinc finger nuclease strategies to target an integrated reporter and two endogenousrat genes, Immunoglobulin M (IgM) and Rab38. The mutations are induced at high frequency (animals carry 25 to 100% disruption at the target locus) by standard microinjection of DNA or RNA molecules encoding specific Zinc Finger Nucleases. These mutations are faithfully and efficiently transmitted through the germline. Quoting the authors: “…Our data demonstrate the feasibility of targeted gene disruptionin multiple rat strains within 4 months time, paving the wayto a humanized monoclonal antibody platform and additional humandisease models.”
The Istituto di Biologia Cellulare of Consiglio Nazionale delle Ricerche-“A. Buzzati-Traverso” Campus and The Jackson Laboratory offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries that will be held in Monterotondo (Italy), on November 23-26, 2009. This Course is financially supported by the EU FP7 Capacities Specific Program, through the European Mouse Mutant Archive (EMMA).
Deadline for submitting applications: October 15, 2009
Additional information about this cryopreservation course: INFORMATION
On behalf of the ISTT, the TT2010 Organizing Committee and its chair, Dr. Boris Jerchow, it is my pleasure to launch the TT2010 meeting web site, containing all currently available information regarding the 9th Transgenic Technology Meeting that will be held in Berlin, Germany, at the MDC, on March 22-24, 2010.
A limited number of hotel rooms have been reserved for the TT2010 meeting at reduced prices, please check the list of pre-arranged TT2010 conference hotels. In order to ensure that your hotel reservation can be made as required we recommend applying as early as possible together with your registration and abstract submission. Rooms will be reserved on a first come – first serve basis.
The 5th workshop on Innovative Mouse Models 2009 (IMM2009) was held recently, on June 25-26, at the Leiden University Medical Center (LUMC) in Leiden (The Netherlands) with the co-sponsorship of the ISTT. Marian van Roon (AMC, Netherlands, Netherlands), member of ISTT, formed part of the Local Organizing Committee, that also included Jos Jonkers, Paul Krimpenfort, Werner Mueller, Els Robanus-Maandag, Hein te Riele and Sjef Verbeek. The workshop program included the following keynote invited speakers: Anton Berns, Adam Dupuy, Andras Nagy, Barry Rosen, Austin Smith, Francis Stewart, Lars Zender. This workshop was most successful. At the meeting, Boris Jerchow, the chair of the TT2010 meeting Organizing Committee, and member of the ISTT council, announced and advertised this forthcoming 9th TT Meeting, that will be held at MDC, in Berlin, Germany, on March 22-24, 2010.