Collection of videos of animal transgenesis at the ISTT

58 videos availbale at the ISTT
58 videos available at the ISTT

Currently, we have 58 videos on different aspects of animal transgenesis, kindly and generously shared by various ISTT Members. Videos are available within the members-only area of the ISTT web site. If you are not yet ISTT Member, please consider registering to our Society.

The current list of ISTT videos include:

1) Pronuclear microinjection of DNA into a sheep fertilised oocyte

2) Microinjection of murine ES cells into a mouse blastocyst

3) Nuclear transfer in mouse embryos. Nucleus of a blastomer into a enucleated metaphase II oocyte

4) Intracytoplasmic Sperm Injection (ICSI). Handling thawn (dead) sperm heads incubated with DNA solution (collecting heads and preparing for microinjection) as reported in Moreira et al. 2006

5) Intracytoplasmic Sperm Injection (ICSI) Microinjecting (piezo) sperm heads into metaphase II oocytes as reported in Moreira et al. 2006

6) Pronuclear microinjection of DNA into a mouse fertilised oocyte

7) Pronuclear microinjection of DNA into a mouse fertilised oocyte II

8 ) Pronuclear microinjection of DNA into a mouse fertilised oocyte III

9) Pronuclear microinjection of DNA into a mouse fertilised oocyte IV

10) Pronuclear microinjection of DNA into a mouse fertilised oocyte V

11) Pronuclear microinjection of DNA into a mouse fertilised oocyte VI

12) Pronuclear microinjection of DNA into a mouse fertilised oocyte VII

13) Pronuclear microinjection of DNA into a mouse fertilised oocyte VIII

14) Pronuclear microinjection of DNA into a mouse fertilised oocyte IX

15) Pronuclear microinjection of DNA into a mouse fertilised oocyte X

16) ES cell microinjection into a mouse blastocyst I

17) ES cell microinjection into a mouse blastocyst II

18) ES cell microinjection into a mouse blastocyst III

19) ES cell microinjection into a mouse blastocyst IV

20) Picking up mouse ES cells into a microinjection pipette

21) Loading a pipette with mouse embryos

22) Oviduct (infundibulum) transfer of mouse embryos

23) Intracytoplasmic Sperm Injection (ICSI) Microinjecting (piezo) sperm heads into metaphase II oocytes

24) Removing tails from sperm heads with a piezo

25) Fill – Lentivrus injection procedure (loading lentivirus and cellular debris particles into the pipette)

26) Subzonal Injection. Lentivrus injection procedure (releasing lentivirus and cellular debris particles in the perizonal space, under the zona pellucida)

27) Discharge – Lentivirus injection (releasing lentivirus and cellular debris particles out of the pipette)

28) 2-cell mouse embryos post IVF

29) How to grab a mouse with one hand? I

30) How to grab a mouse with one hand? II

31) How to grab a mouse with one hand? III

32) Mouse intraperitoneal (IP) injection

33) Application of ear tags to mice for identification purposes I (usually done at weaning)

34) Application of ear tags to mice for identification purposes II (usually done at weaning)

35) Mouse tail biopsy using a home-made mouse restraint device and a sharp red-hot blade attached to a soldering tool for cutting and wound cauterisation purposes I (usually done at weaning)

36) Mouse tail biopsy using a home-made mouse restraint device and a sharp red-hot blade attached to a soldering tool for cutting and wound cauterisation purposes II (usually done at weaning)

37) Mouse euthanasia by cervical dislocation I

38) Mouse euthanasia by cervical dislocation II

39) Visual phenotyping of spatial acuity in mice with the optomotor test as described in Lavado et al. 2006

40) Visual phenotyping of spatial acuity in mice with the optomotor test. Cylinder turns left, albino mouse turns random because it cannot see, as described in Lavado et al. 2006.

41) Visual phenotyping of spatial acuity in mice with the optomotor test. Cylinder turns right, albino mouse turns random because it cannot see, as described in Lavado et al. 2006

42) Visual phenotyping of spatial acuity in mice with the optomotor test. Cylinder turns left, pigmented mouse turns left too because it can seem as described in Lavado et al. 2006

43) Visual phenotyping of spatial acuity in mice with the optomotor test. Cylinder turns right pigmented mouse turns right too because it can see, as described in Lavado et al. 2006

44) Mouse ICSI using the XYClone Laser

45) ES-cell injection into mouse 8-cell embryos using the XYClone Laser

46) ES-cell injection into mouse blastocysts using the XYClone Laser

47) Mouse ICM xenogenic-free excission using the XYClone Laser

48) Bovine embryo biopsy using the XYClone Laser

49) Bovine ICSI using the XYClone Laser

50) Mouse vasectomy (abdominal)

51) Oviduct transfer upstream of the ampulla. (Oviduct “hole in the wall” transfer technique) Transfer between the infundibulum and the ampulla

52) Laser-assisted ES cell injection into a mouse 8 cell embryo using the OCTAX Laser Shot system (MTG)

53) Mouse vasectomy (scrotal)

54) Non-Surgical Embryo Transfer Device NSET (ParaTechs)

55) Flushing a mouse oviduct

56) Washing fertilised oocytes after IVF (transferring from HTF to KSOM)

57) Stained mid-gestation lacZ reporter mouse embryos (Myf5, Kit, Pax3 and Dct lacZ reporter mouse embryos are shown in this order, audio available)

58) ES cell injection into mouse 8-cell embryos

ISTT Forum has been moved to the MEMBERS-ONLY area

ISTT Forum
ISTT Forum

The ISTT Forum has been moved to the MEMBERS-ONLY area of the ISTT web site. This measure has been decided after the numerous attempts from unrelated users, spammers, robots, spiders and all sorts of attacks willing to register and use the forum for unacceptable purposes. Since we believe that the ISTT Forum is an important ISTT resource using a substantial amount of administrative webmaster tasks it will be now transferred to a protected environment and thus, only accessible to ISTT Members. All non-ISTT Members, previously registered as visitors in the ISTT forum, are kindly invited to join the ISTT to benefit from this and many other features available only to ISTT Members. Thanks for your collaboration and understanding. Please, don’t hesitate to contact us if you have any question, comment or suggestion. ISTT